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Calcium-binding proteins in the oro-facial regions : Distribution and functional implication

Research Project

Project/Area Number 09671849
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOsaka University

Principal Investigator

WAKISAKA Satoshi  Osaka University Faculty of Dentistry Associate Professor, 歯学部, 助教授 (40158598)

Co-Investigator(Kenkyū-buntansha) TABATA Makoto  Osaka University Faculty of Dentistry Research Associate, 歯学部, 助手 (20243248)
NAGASE Yoshitaka  Osaka University Faculty of Dentistry Research Associate, 歯学部, 助手 (50252698)
OGAWA Yuzo  Osaka University Faculty of Dentistry Associate Professor, 歯学部, 助教授 (10135725)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordscalcium-binding proteins / carbonic anhydrase / taste buds / periodontal ligament / Ruffini endings / dental pulp / regeneration / development / カルシウム結合蛋白
Research Abstract

Calcium-binding proteins (CaBP) are a family of proteins with high affinity for binding calcium ions and may participate in important processes by changing the intracellular calcium concentrations.In the present project, we investigated the distribution of CaBP such as calbindin D28k (CB), calretinin (CR) and S1OObeta in the dental pulp, periodontal ligament and taste buds during development and regeneration.In addition, since some CaBP co-localized with carbonic anhydrase isozyme II (CA II) in certain neurons, we examined the distribution of CA II.
In the dental pulp, CB was localized in the axons of dental pulp of the rat molar. Some nerve fibers penetrating the predentin are immunoreactive for CB.S1OObeta, which has been long believed to be a marker for Schwann cells, is also present in the axons.It is known that periodontal ligament abundantly contains Ruffini endings.The axon terminals of Ruffini endings contain CB, CR, S1OObeta and CA II.During development and regeneration of Ruffini endings, expression of CR was delayed.In the taste buds, type III cells (gustatory cells) contained CB, while type I cells (supporting cells) contained CA II.The expression of CB in the taste buds during development and regeneration delayed compared with appearance of the taste buds.In the periodontal ligament of the adult animal, CB was localized in the certain cells of the epithelial rest of Malassez, cells between root dentin and cementum and some fibroblasts.During root formation, some epithelial cells began to express CB after the fragmentation of the Hertwig's epithelial sheath.Expression of CB in the periodontal fibroblasts started after the commencement of the occlusion.These evidences suggest that CaBP have important roles in the mechano-transduction of periodontal Ruffini endings, maintenance of taste buds cells, and survival of epithelial cells and fibroblasts in the periodontal ligament.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] K.Ochi et al.: "Immunohistochemical localization of calbindin D28k in the periodontal Ruffini endings of rat incisors" Neurocience Letters. 228. 195-198 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ochi et al.: "Calretinin-like immunoreactivity in the Ruffini endings,slowly adapting mechanoreceptors,of the periodontal ligament of the rat incisor" Brain Research. 769. 183-187 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Miyawaki et al.: "Calbindin D28k-like immunoreactive nerve fibres in the predentine of rat molar teeth" Archives of Oral Biology. 42. 773-777 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Miyawaki et al.: "Calbindin D28k-like immunoreactivity in the developing and regenerating circumvallate papilla of the rat" Cell and Tissue Research. 291. 137-143 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ochi et al.: "Carbonic anhydrase isozyme II immunoreactivity in the mechanoreceptive Ruffini endings of the periodontal ligament of the rat incisor" Brain Research. 779. 276-279 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Hiroshima et al.: "Calretinin-like immunoreactivity in the regenerating periodontal Ruffini endings of the rat incisor following injury to the inferior alveolar nerve" Brain Research. 807. 218-221 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Nakakura-Ohshima et al.: "Immunocytochemical detection of S-100β in the periodontal Ruffini endings in the rat inciosor" Neuroscience Letters. 258. 163-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Atsumi et al.: "Immunohistochemical localiation of S-100β in the dental pulp of the rat molar" Brain Research. 818. 515-519 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Daikoku et al.: "Immunohistochemical localization of carbnic anhydrase isozyme II in the gustatory epithelium of the adult rat" Chemical Senses. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Asahito et al.: "Postnatal expression of calretinin-like immunoreactivity in the periodontal Ruffini endings in the rat incisor : A comparison with protein gene product 9.5(PGP9.5)-immunoreactivity" Archives of Histology and Cytology. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Onishi et al.: "Immunohistochemical localization of calbindin D28k during the root formation of the rat molar teeth" Cell and Tissue Research. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Maeda et al.: "Ruffini ending as an essential mechanoreceptor in periodontal ligament-Its morphology,cytochemical feature,regeneration and development" Critical Reviews in Oral Biology and Medicine. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ochi et al.: "Immunohistochemical localization of calbindin D28k in the periodontal Ruffini endings of rat incisors" Neuroscience Letters. 228. 195-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ochi et al.: "Calretinin-like immunoreactivity in the Ruffini endings, slowly-adapting mechanoreceptors." Brain Research. 768. 183-187 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Miyawaki et al.: "Calbindin D28k-like immunoreactive nerve fibers in the predentine of rat molar teeth" Archives of Oral Biology. 42. 773-777 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Miyawaki et al.: "Calbindin D28k-like immunoreactivity in the developing and regenerating circumvallate papilla of the rat" Cell and Tissue Research. 291. 81-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ochi et al.: "Carbonic anhydrase isozyme II in the mechanoreceptive Ruffini endings of the periodontal ligament of the rat incisor" Brain Research. 779. 276-279 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Hiroshima et al.: "Calretinin-like immunoreactivity in the regenerating periodontal Ruffini endings of the rat incisor following Injury to the inferior alveolar nerve" Brain Research. 807. 218-221 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Nakakura-Ohshima et al.: "Immunocytochemical detection of S-100beta in the periodontal Ruffini endings in the rat incisor" Neuroscience Letters. 258. 163-166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Atsumi et al.: "Immunohistochemical localization of S-100beta in the dental pulp of the rat molar" Brain Research. 818. 515-519 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Daikoku et al.: "Immunohistochemical localization of carbonic anhydrase isozyme II in the gustatory epithelium of the adult rat" Chemical Senses. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Asahito et al.: "Postnatal expression of calretinin-like immunoreactivity in the periodontal Ruffini endings in the rat incisor : A comparison with protein gene product 9.5 (PGP9.5)-immunoreactivity" Archives of Histology and Cytology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Onishi et al.: "Immunohistochemical localization of calbindin D28k during the root formation of the rat molar teeth" Cell and Tissue Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Maeda et al.: "Ruffini ending as an essential mechanoreceptor in periodontal ligament-Its morphology, cytochemical feature, regeneration and development-" Critical Reviews in Oral Biology and Medicine. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ochi et al.: "Carbonic anhydrase isozyme II immunoreactivity in the mechanoreceptive Ruffini endings of the periodontal ligament of the rat incisor" Brain Research. 779. 276-276 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Hiroshima et al.: "Calretinin-like immunoreactivity in the regenerating periodontal Ruffini endings of the rat incisor following injury to the inferior alveolar nerve" Brain Research. 807. 218-221 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Nakakuma-Ohshima et al.: "Immunocytochemical detection of S-100β in the periodontal Ruffini endings in the rat inciosor" Neuroscience Letters. 258. 163-166 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Atsumi et al.: "Immunocytochemical localiation of S-100β in the dental pulp of the rat molar" Brain Research. 818. 515-519 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Daikoku et al.: "Immunohistochemical localization of carbnic anhydrase isozyme II in the gustatory epithelium of the adult rat" Chemical Senses. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Asahito et al.: "Postnatal expression of calretinin-like immunoreactivity in the periodontal Ruffini endings in the rat incisor:A comparison with protein gene product 9.5(PGP9.5)-immunoreactivity" Archives of Histology and Cytology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Onishi et al.: "Cell and Tissue Research" Immunohistochemical localization of calbindin D28k during the root formation of the rat molar teeth, 1999 ((in press))

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Maeda et al.: "Critical Review in Oral Biology and Medicine" Ruffini ending as an essential mechanoreceptor in periodontal ligament-Its morphology,cytochemical feature,regeneration and development, 1999 ((in press))

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Ochi et al.: "Immunohistochemical localization of calbindin D28k in the periodontal Ruffini endings of rat incisors" Neurocience Letters. 228. 195-198 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Ochi et al.: "Calretinin-like immunoreactivity in the Ruffini endings,slowly adapting mechanoreceptors,of the periodontal ligament of the rat incisor" Brain Research. 769. 183-187 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Miyawaki et al.: "Calbindin D28k-like immunoreactive nerve fibres in the predentine of rat molar teeth" Archives of Oral Biology. 42. 773-777 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Miyawaki et al.: "Calbindin D28k-like immunoreactivity in the developing and regenerating circumvallate papilla of the rat" Cell and Tissue Research. 291. 137-143 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Wakisaka et al.: "Correlation between neuropeptide Y and calcium binding proteins in the trigeminal ganglion following peripheral axotomy to the inferior alveolar nerve" Regulatory Peptides. 71. 231 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Ochi et al.: "Carbonic anhydrase isozume II immunoreactivity in the mechanoreceptive Ruffini endings of the periodontal ligament of the rat incisor" Brain Research. 779. 276-279 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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