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Molecular cloning of the receptors for chondrosarcoma-derived chondrocyte growth factor Ecogenin/CTGF

Research Project

Project/Area Number 09671893
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOkayama University

Principal Investigator

NAKANISHI Tohru  Okayama University Dental School, Associate Professor, 歯学部, 助教授 (30243463)

Co-Investigator(Kenkyū-buntansha) OHYAMA Kazumi  Okayama University Dental School, Research Assistant, 歯学部, 教務員 (00253021)
HATTORI Takako  Okayama University Dental School, Instructor, 歯学部, 助手 (00228488)
TAKIGAWA Masaharu  Okayama University Dental School, Professor, 歯学部, 教授 (20112063)
INOUE Miho  Okayama University Dental School, Research Assistant, 歯学部, 教務員 (20271059)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsdifferential display / connective tissue growth factor (CTGF) / crosslink / affinity chromatography / MAP kinase / proteoglycan / integrin / tyrosine kinase / CTGF / レセプター / 内軟骨性骨化 / ペプチドマッピング / 軟骨細胞 / 結合組織増殖因子(CTGF) / Scatchard解析 / crosslink / PDGF / FGF
Research Abstract

(1) Connective tissue growth factor (CTGF) was cloned from a chondrocyte-derived chondrocytic cell line, HCS-2/8 by differential display-PCR.
(2) Recombinant CTGF (rCTGF) stimulated the proliferation, maturation and differentiation of chondrocytes.
(3) Two types of receptors for CTGF were found on a chondrocyte-derived chondrocytic cell line, HCS-2/8. The receptor with high affinity was supposed to be cell adhesion molecules including integrins. The receptor with low affinity was supposed to be extracellular matrix compounds including proteoglycans.
(4) The inhibitory experiments using signal inhibitors showed that intercellular signal transduction in HCS-2/8 cells caused by the stimulation of CTGF was mediated by MAP kinase-pathways including MEK and ERK.
(5) CTGF-binding proteins were purified from membrane fractions and cytoplasmic fractions of HCS-2/8 cells with CTGF-conjugated affinity chromatography. As a result, four binding proteins (34, 44, 66 kDa from membrane fractions 50 kDa from cytoplasmic fractions) were purified from HCS-2/8 cells. The expression of these four proteins were regulated by the stimulation of CTGF, suggesting that they were functionally associated with CTGF.
(6) The cDNA of tyrosine kinase-type receptors were cloned from HCS-2/8 cells using degenerate primers corresponding to the consensus sequences between tyrosine kinase-domains. The sequence analysis of these cDNA revealed that there were several types of tyrosine kinase-receptors including novel receptors in HCS-2/8 cells. The expression of these receptors were regulated by the stimulation of CTGF. In addition, CTGF-producing cells showed high level of expression of these receptors. These results suggest that they were functionally associated with CTGF.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Nakanishi, T.: "Cloning of mRNA preferentially expressed in chondrocytes by differential display PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA"Biochem. Biophys. Res. Commun.. 234. 206-210 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishida, T.: "Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)"Biochem. Biophys. Res. Commun.. 247. 905-909 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamatani, T.: "Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia"Biochem. Biophys. Res. Commun.. 251. 748-752 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi, H.: "Increased expression of connective tissue growth factor in the infarct zone of experimentally induced myocardial infarction in rats"J. Mol. Cell Cardiol.. 30. 2411-2422 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimo, T.: "Inhibition of endogenous expression of connective tissue growth factor by its antisense oligonucleotide and antisense RNA suppresses proliferation and migration of vascular endothelial cells"J. Biochem.. 124. 13-140 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 滝川正春: "内軟骨性骨形成に最も重要な新規成長因子CTGF"細胞工学. 17. 357-362 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mori, T.: "Role and interaction of connective tissue growth factor with transforming growth factor-beta in presistent fibrosis : A Mouse fibross model"J. Cell. Physiol.. 181. 153-159 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota, S.: "Involvement of cis-acting repressive element(a9 in the 3'-untranslated region of human connective tissue growth factor gene"FEBS Lett.. 450. 84-88 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kondo, Y.: "Immunohistochemical localization of connective tissue growth factor in the rat central"Brain Res.. 834. 146-151 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimo, T.: "Connective tissue growth factor induces the proliferation, migration and tube formation of vascular endothelial cells in vitro and angiogenesis in vivo"J. Biochem.. 126. 137-145 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 中西 徹: "結合組織成長因子CTGF/Hcs24の生理機能"生化学. 71. 429-432 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture"Endocrinology. 141. 264-273 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Cloning of a mRNA preferentially expressed in chondrocytes by differential display PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA"Biochem. Biophys. Res. Commun.. 234. 206-210 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimo, T.: "Inhibition of endogenous expression of connective tissue growth factor by its antisense oligonucleotide and antisense RNA suppresses proliferation and migration of vascular endothelial cells"J. Biochem.. 124. 130-140 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishida, T.: "Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)"Biochem. Biophys. Res. Commun.. 247. 905-909 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamatani, T.: "Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia"Biochem. Biophys. Res. Commun.. 251. 748-752 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi, H.: "Increased expression of connective tissue growth factor in the infarct zone of experimentally induced myocardial infraction in rats"J. Mol. Cell Cardiol. 30. 2411-2422 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota, S.: "Involvement of cis- acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene"FEBS Lett.. 450. 84-88 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kondo, Y.: "Immunohistochemical localization of connective tissue growth factor in the rat central nervous system"Brain Res.. 834(1-2). 146-151 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimo, T.: "Connective tissue growth factor induces the proliferation, migration and tube formation of vascular endothelial cells in vitro and angiogenesis in vivo"J. Biochem.. 126(1). 137-145 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mori, T.: "Role and interaction of connective tissue growth factor with transformation growth factor-beta in persistent fibrosis : A mouse fibrosis model"J. Cell Physol.. 181. 153-159 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakanishi T.: "Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture"Endocrinology. 141(1). 264-273 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota S.: "Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene"FEBS Lett.. 450. 84-88 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kondo Y.: "Immunohistochemical localization of connective tissue growth factor in the rat central nervous system"Brain Res.. 834. 146-151 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimo T.: "Connective tissue growth factor induces the proliferation, migration and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo"J. Biochem.. 126. 137-145 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mori T.: "Role and interaction of connective tissue growth factor with transforming growth factor-beta in persistent fibrosis: A mouse fibrosis model"J. Cell Physiol.. 181. 153-159 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakanishi T.: "Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture"Endocrinol.. 141. 264-273 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimo, T.: "Inhibition of endogenous expression of connective tissue growth factor by its antisense oligonucleotide and antisense RNA suppresses proliferation and migration of vascular endothelial cells." J. Biochem.124. 130-140 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishida, T.: "Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)" Biochem. Biophys. Res. Commun.247. 905-909 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamatani, T.: "Establishment of the enzymelinked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia." Biochem. Biophys. Res. Commun.251. 748-752 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohnishi, H.: "Increased expression of connective tissue growth factor in the infarct zone of experimentally induced myocardial infarction in rats." J. Mol. Cell Cardiol.30. 2411-2422 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中西徹: "結合組織成長因子CTGF/Hcs24の生理機能。" 生化学. 印刷中.

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakanishi,T.: "Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA" Biochem.Biophys.Res.Commun.234. 206-210 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 滝川正春: "内軟骨性骨形成に最も重要な新規成長因子CTGF" 細胞工学. 17. 357-362 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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