|Budget Amount *help
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2000 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1999 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1998 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1997 : ¥2,000,000 (Direct Cost : ¥2,000,000)
It was made to intervene artificial periodontal ligament using human periodontal ligament derivation culture cell in the implant circumference, and the human periodontal ligament derivation cell would be continuously cultivated by the natural tooth for the purpose of the reappearance of the function, and the basic study on cell culture was carried out. As a result of repeating the examination of the picking condition, success rate improved the primary culture of the periodontal ligament fibroblast-like cells collected from the tooth root plane. As a result, the subculture that completed and stabilized the recultivation was difficult.
The culture of the periodontal ligament cell in the monolayer became possible. There is the report, however, the construdion of the periodontal ligament is difficult by transplantation animal in the periodontal ligament cell of the monolayer. The culture method which is a three-dimensional environment equal to the condition of the organism. It is called a culture method in collagen gel, it was examined that this method was tried. However, it does not succeed, since the sufficient cell population is not obtained. The clinical application is considered, it is good condition to use the cell in the time with active cell proliferation and differentiation induction potency. Cell adherent living body material efficiently and important that multiplication spedalization can be carried out. Therefore, a cell cycle and cell multiplication ability were examined. The cells which extracts a the periodontal ligament cells were very small quantity. In order to carry out clinical application widely, it was thought that it was important because of development continuation of this research of establishment of the cell culture system of in vitro and the saving method.