|Budget Amount *help
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2000 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1999 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1998 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1997 : ¥2,000,000 (Direct Cost : ¥2,000,000)
It was made to intervene artificial periodontal ligament using human periodontal ligament derivation culture cell in the implant circumference, and the human periodontal ligament derivation cell would be continuously cultivated by the natural tooth for the purpose of the reappearance of the function, and the basic study on cell culture was carried out. As a result of repeating the examination of the picking condition, success rate improved the primary culture of the periodontal ligament fibroblast-like cells collected from the tooth root plane. As a result, the subculture that completed and stabilized the recultivation was difficult.
The culture of the periodontal ligament cell in the monolayer became possible. There is the report, however, the construdion of the periodontal ligament is difficult by transplantation animal in the periodontal ligament cell of the monolayer. The culture method which is a three-dimensional environment equal to the condition of the organism. It is called a
culture method in collagen gel, it was examined that this method was tried. However, it does not succeed, since the sufficient cell population is not obtained. The clinical application is considered, it is good condition to use the cell in the time with active cell proliferation and differentiation induction potency. Cell adherent living body material efficiently and important that multiplication spedalization can be carried out. Therefore, a cell cycle and cell multiplication ability were examined. The cells which extracts a the periodontal ligament cells were very small quantity. In order to carry out clinical application widely, it was thought that it was important because of development continuation of this research of establishment of the cell culture system of in vitro and the saving method.
これまでに単層での歯根膜細胞の培養は可能となったが、単層の歯根膜細胞では動物に移植した実験で歯根膜組織の構築は困難であるとの報告がみられることから、実験室内で生体の条件に近いとされる細胞の3次元的環境での培養法であるコラーゲンゲル内培養法を試みることを検討したが十分な細胞数が得られず、未だ成功していない。臨床応用を考慮すると、活発な細胞増殖と分化誘導能を有している時期の細胞を用いることが適当で、細胞が生体材料に効率よく接着し、増殖分化できることが重要と思われるため、細胞周期、細胞増殖能について検討した。歯根膜細胞は採取する細胞が微量であるため、広く臨床応用していくためには、in vitroの細胞培養系と保存法の確立が本研究の発展継続のために重要であると思われた。 Less