|Budget Amount *help
¥3,300,000 (Direct Cost : ¥3,300,000)
Fiscal Year 1998 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 1997 : ¥2,100,000 (Direct Cost : ¥2,100,000)
Kinetics of cell growth and degradation of chlorinated organic compounds were examined and established experimentally or theoretically for the purpose of bioremediation of underground water contaminated by chlorinated organic compounds. These summary are shown as follows :
In the biodegradation of 1,2-dichloroetane by Pseudomonas putida IAM 1002, 1,2-dichloroetane of high concentration to give 2000 ppm was rapidly degraded by means of activated cells with ethanol. The degradation fractions of 1,2-dichloroetane ranging in concentration from 100 to 2000 ppm were shown over 90% in a week and the residual 1,2-dichloroetane could be reduced to 10 〜 30 ppm. In the qualitative identification of degradation products, it was proved 1,2-dichloroetane were degraded up to inorganic chloride ions and made nontoxic via 2-chloroethanol, chloroacetaldehyde and monochloroaceticacid.
In the biodegradation of 1,2-dichloroetane by Acinetobacter calcoaceticus IAM 12580, 1,2-dichloroetane of 50 〜 1000 ppm was used as the only substrate and rapidly degraded without activation of cells by ethanol. The degradation fractions of 1,2-dichloroetane were shown over 90% and the residual 1,2-dichloroetane were less than 40 ppm to give the environmental standard. From these results, the effectiveness for bioremediation of 1,2-dichloroetane by this bacteria was verified. Furthermore, Monod's equation was applicable to the cell growth of this bacteria and the kinetics of cell growth and degradation of chlorinated organic compounds were formulated using the estimated growth parameter of cell yield maximum specific growth rate and saturation constant.