• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biased distribution of pathogenic Entamoeba histolytica

Research Project

Project/Area Number 10041202
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionNagasaki University

Principal Investigator

KANBARA Hiroji  Nagasaki University, Inst. of Trop. Med., Professor, 熱帯医学研究所, 教授 (20029789)

Co-Investigator(Kenkyū-buntansha) TACHIBANA Hiroshi  Tokai University, School of Med., Associate Professor, 医学部, 助教授 (10147168)
YANAGI Tetsuo  Nagasaki University, Inst. of Trop. Med., Research Associate, 熱帯医学研究所, 助手 (10174541)
UEMURA Haruki  Nagasaki University, Inst. of Trop. Med., Assistant Professor, 熱帯医学研究所, 講師 (60184975)
RIVERA Windell l.  Tokai University, School of Med., Postdoctoral Fellow, 医学部, 特別研究員
RIVERA Windell L.  東海大学, 医学部, 特別研究員
RIVERA Pilar  College of Public Health, U. P. Manila, Associate
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsEntamoeba histolytica / Entamoeba dispar / pathogenic species / transmission mode / Philippines / PCR / cyst / PCR / 病原性 / PCR診断 / アメーバ疫学調査
Research Abstract

Recently, Entamoeba histolytica has been recognized to consist of a pathogenic species, E. histolytica and a non-pathogenic species, E. dispar. This means that the conventional method using microscopic examination of fecal samples can not determine prevalence of pathogenic E. histolytica that is more important from the viewpoint of public health. Since the PCR method using extracted DNA from cysts has been shown to be useful for differentiation of E. histolytica from E. dispar, it was used in combination with the microscopic method to clarify the difference of the transmission mode between two species at various parts of Luzon in the Philippines. The following results were obtained.
1) The prevalence of two species was not over 15% in all the places examined, although the prevalence of helminthic infection reached nearly 70% in the slum area. This indicated that the transmission mode of Entamoeba is less effective than some helminths.
2) The prevalence of a pathogenic species, E. histolytica was less than a half of that of a non-pathogenic species, E. dispar in all the areas examined.
3) Both species showed a with variety of prevalence according to places. If the condition of public health is similar, the colder climate provides the better condition for transmission of Entamoeba.
4) The distribution of the pathogenic species was more varied than the non-pathogenic. Transmission from a human to a human is inferred to be maintained mainly by intimate contact in a family. Consequently the highest prevalence was found in the slum area.
5) Higher prevalence was found in children aged from 5 to 14 years, and in women than in men. This may be caused by differences of their living habits.
6) High prevalence of E. dispar was found in Japanese monkeys at the Takasaki mountain, Oita prefecture, Japan. This indicated that monkeys are more fitting hosts for E. dispar than humans.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Rivera WL et al.: "Detection of Entamoeba diapar DNA in macaque feces by polymerase chain reaction"Parasitology Resaerch. 85. 493-495 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rivera WL et al.: "Application of the polymerase chain reaction (PCR) on the epidemiology of Entamoeba histolytica and Entamoeba dispar Infections"Tokai Journal of Experimental and Clinical medicine. 23. 413-415 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rivera WL et al.: "Field Study on the Distribution of Entamoeba histolytica and Entomoeba dispar in the Northern Philippines as Detected by the Polymerase Chain Reactions"the american Journal of Tropical Medicine and Hygiene. 59. 916-921 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rivera WL, and Kanbara H.: "Detection of Entamoeba dispar DNA in macaque feces by polymerase chain reaction"Parasitology Research. 85. 493-495 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rivera WL, Tachibana H. and Kanbara H.: "Application of the polymerase chain reaction (PCR) on the epidemiology of Entamoeba histolytica and Entamoeba dispar Infections"Tokai Journal of Experimental and Clinical Medicine. 23. 413-415 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rivera WL, Tachibana H. and Kanbara H.: "Field Study on the Distribution of Entamoeba histolytica and Entamoeba dispar in the Northern Philippines as Detected by the Polymerase Chain Reaction"The American Journal of Tropical Medicine and Hygiene. 59. 916-921 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rivera W.L.et al: "Detection of Entamoeba dispar DNA in macaque feces by polymerase chain reaction"Parasitology Research. 85. 493-495 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Rivera W.L.,et al: "Application of the polymerase chain reaction (PCR) on the epidemiology of Entamoeba histolytica and Entamoeba dispar Infections"Tokai Journal of Experimental and Clinical Medicine. 23(6). 413-415 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Windell L.Rivera et al: "Detection of Entamoeba dispar DNA in macaque feces by polymerase chain reaction." Parasitology Research. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Windell L.Rivera et al.: "Application of the polymerase chain reaction (PCR) on the epidemiology of Entamoeba histolytica and Entamoeba dispar Infections." Tokai Journal of Experimental and Clinical Medicine. (in press). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Windell L.Rivera et al.: "Field Study on the Distribution of Entamoeba histolytica and Entamoeba dispar in Northern Philippines as Detected by Polymerase Chain Reaction." The American Journal of Tropical Medicine and Hygiene. 59. 916-921 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Windell L.Rivera et al.: "Differentiation of Entamoeba histolytica and E.dispar DNA from cysts present in stool specimens by polymerase chain reaction : its field application in the Philippines." Parasitology Research. 82. 585-589 (1996)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi