|Budget Amount *help
¥4,800,000 (Direct Cost : ¥4,800,000)
Fiscal Year 1999 : ¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1998 : ¥2,600,000 (Direct Cost : ¥2,600,000)
Collaboration was done with Dr.Verma (SALK Institute, U.S.A.), Dr.O'Brien (NCI, U.S.A.), Dr. Durandy (INSERM, France) and their colleagues on a cytokine, SDF-1, which is the natural ligand for CXCR4, the coreceptor for T tropic HIV virus.
1. To understand molecular mechanism of our finding that AIDS onset is delayed in the persons who bear homozygous alleles of SDF1 gene AIDS delaying type polymorphysm, the blood SDF-1 concentration in each individuals bearing each genotype was measured. For that, new measuring system named SDF-1 TR-FIA was developed in collaboration with Dr.Matsumoto (Waseda Univ./CREST) and blood samples were collected by Dr.O'Brien. The measuring work is still on going.
2. In our SDF-1 expression system in vivo, CXCR4 on CD4ィイD1+ィエD1 cells were down-modulated in the presence of excess amount of SDF-1, showing the molecular mechanism of the delay.
3. We are screening additional polymorphic site linked with the SDF1 gene AIDS delaying type polymorphysm.