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Role of Cardiovascular-related Peptides in the Brain : Genetic, Molecular Biological, Morphological and Physiological Aspects.

Research Project

Project/Area Number 10044332
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

YAMASHITA Hiroshi  University of Occupational and Environmental Health, School of Medicine, Department of Physiology, Professor, 医学部, 教授 (00030841)

Co-Investigator(Kenkyū-buntansha) INENAGA Kiyotoshi  Kyushu Dental College, Department of Physiology, Professor, 歯学部, 教授 (90131903)
UETA Yoichi  University of Occupational and Environmental Health, School of Medicine, Department of Physiology, Lecturer, 医学部, 講師 (10232745)
SHIBUYA Izumi  University of Occupational and Environmental Health, School of Medicine, Department of Physiology, Associate Professor, 医学部, 助教授 (50162649)
寒川 賢治  国立循環器病センター, 生化学部, 部長 (00112417)
TRIBOLLET El  ジュネーブ大学, 医学部, 講師
KOIZUMI Kiyo  ニューヨーク州立大学, 医学部, 教授
KOW Lee Ming  ロックフェラー大学, 神経生物&行動学, 助教授
VALE Wylie  ソーク研究所, ペプチド生物学研究室, 教授
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsVasopressin / Adrenomedullin / Oxytocin / Orexin / Leptin / Urocortin / PACAP / アドレノメデユリン / 循環 / ペプチド / 視床下部 / 室傍核 / 視索上核 / イオンチャネル / 学習
Research Abstract

The purpose of the present study was to investigate the role of cardiovascular-related peptides in the central nervous system (CNS), using genetic, molecular biological, morphological and physiological approaches. These approaches focused on the integrative investigation of the role from the genetics to behavior of cardiovascular-related peptides in the CNS. The examined cardiovascular-related peptides were vasopressin, adrenomedullin, orexin/hypocretin, leptin, TRH, CRH, urocortin and PACAP. (1) The expression of the vasopressin gene in the hypothalamus was upregulated in genetically polydipsic mice. (2) Adrenomedullin-like immunoreactive cells were distributed in the hypothalamic area, including the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of rats. Central administration of adrenomedullin selectively activated oxytocin-secreting neurons in the PVN and the SON with elevation of plasma oxytocin. (3) Orexin/hypocretin was newly discovered orexigenic peptides. Orexin-pr … More oducing neurons were localized in the lateral hypothalamic area. Their axons project to various regions in the CNS. Central administration of orexin selectively activated hypothalamo-pituitary adrenal axis in rats. Neonatal development of orexin in rats were examined. The expression of the orexin gene was markedly increased 15 and 20 days after birth. (4) The food deprivation induced down-regulation of neuronal nitric oxide synthase gene, TRH gene and CRH gene in the PVN were prevented by replacement with leptin. (5) The immunoreactivity of urocotin-producing cells and fibers in the SON was increased in the SON of hypertensive salt sensitive Dahl rats. (6) Central administration of PACAP activated vasopressin-secreting cells in the PVN and the SON and caused an increase in vasopressin heteronuclear RNA in the PVN and the SON. Kainic acid-induced seizure caused a marked induction of the PACAP gene in the parvocellular division of the PVN of rats. Furthermore, it is important to examine the role of cardiovascular-related peptides in the CNS. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] 澁谷泉. 山下博: "サイトカインによる内分泌反応"CLINICAL NEUROSCIENCE. 16. 60-63 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamashita, H et al.: "Control Mechanisms of Stress and Emotion : Neuroendocrine Based Studies"Elsevier Science B.V.. 359 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamashita, H. Ueta, Y. Nomura, M. Isse, T. Shibuya, I. & Inenaga, K: "Osmotic stress and cardiovascular-related peptides., Control mechanisms of stress and emotion : Neuroendocrime-based studies"Elsevier Science B. V.. 247-255 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 上田 陽一,他: "アドレノデュリンの中枢円分布" 医学のあゆみ. 184 (1). 31-33 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shibuya,I. et al.: "PACAP increases the cytosolic Ca^<2+> concentration and stimulates somatodendritic vasopressin release in rat supraoptic neurons." Journal of Neuroendocrinology. 10 (1). 31-42 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Inenaga,K. et al.: "Glutamatergic synaptic inputs tomouse supraoptic neural in calcium-free medium in vitro." Journal of Neuroendocrinology. 10 (1). 1-7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto,S. et al.: "Centrally Administered Parathyroid Hormone (PTH) -Related Protein (1-34) But Not PTH (1-34) Stimulates Arginine-Vasopressin Secretion and It's Messenger RNA Expression in SON of the Consezous Rat" Endocrinology. 138 (1). 383-388 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Uezono,Y. et al: "Activationof inwardly rectifying K^+ channels by GABA-B receptors expressed in xenopus oocytes." Neuroreport. 9 (4). 583-587 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueta,Y. et al.: "Hypovolemia upregulates the expression of neuronal nitric oxide synthase gene in the paraventricular and supraoptic nucle〓 of rats" Brain Research. 790. 25-32 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 澁谷 泉,他: "サイトカインにより内分泌反応" CLINICAL NEUROSCIENCE. 16 (4). 60-63 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 上田 陽一 他: "一酸化窒素(NO)と神経内分泌系" 脳機能の解明 -21世紀に向けて-. 31. 351-354

    • Related Report
      1998 Annual Research Report
  • [Publications] Hattori,Y. et al.: "Ionotropic and metabotropic glutamate receptor agonist-indmed 〔Ca^<2+>〕 i increase in isolated rat supraoptic neuons." Journal of Neuroendocrinology. 10 (5). 383-389 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Uezono,Y. et al.: "Adrenomedullin increases intracellular Ca^<2+> and inositol 14.5-trisphosphate in human oligodendroglial cell line KGIC." Brain Research. 786. 230-234 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Harayama,N. et al.: "Ingibition of N-and P/Q-type calcium channels by postsynaptic GABAB receptor activation in rat sapraoptic neurobs." Joural of Physiology. 509 (2). 371-383 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Serino,R. et al.: "Upregulation of hypothalamic nitric oxide synthase gene expression in streptozotocin-induced diabetic rats." Diabetologia. 41 (6). 640-648 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueta,Y. et al.: "Neuronal nitric oxide synthase gene expression in human pituitary tumors: a possible association with somatotpoph adenomas and growth normonds-releastng hormore gene expression." Clinical Endocrinology. 49. 29-38 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 上田 陽一,他: "アドレノメデュリンと中枢神経系" Molecular Medicine特集 アドレノメジュリンの基礎と その臨床応用. 35 (8). 998-1005 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka,K. et al.: "Pituitary adenylate cydase-actirating polypeptide causes Ca^<2+> release from rganodinelcaffe the stores through a novel pathway independent of both inositol triphosphates and cyclic" Journa of Neurochemistry. 70. 1652-1661 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shibuya,I. et al: "Patch-clamp analysis of the mechanism of PACAP-induced excitation in rat supraoptic neurons." Joural of Neuroendocrinology. 10 (10). 759-768 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wang,U. -F. et al.: "Inhibition of spontaneous inhibitory postsynaptic currents (IPSC) by noradrenaline in rat supraoptic neurons through presynaptic α2-adrereceptor" Brain Research. 807. 61-69 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ibrahim,N. et al.: "GABA_B receptor-mediated ingibition of spontaneous action potential discharge in rat supraoptic neurons." Brain Research. 813. 88-96 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka,K. et al: "Inhibition of voltage-dependent Ca^<2+> channels by prostagraninte in rat melanotrophs." Endocrinology. 139 (12). 4801-4810 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Setiadji,V.S.: "Actions of prostaglandin E_<> on rat supraoptic neurons" Journal of Neuro endocrinology. 10 (12). 927-936 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shibuya,I. et al.: "Evidence that multiple P2X purinoceptors are functionally expressed in rat supraoptic neurons." Journal of Physiology. 514. 351-367 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Date,Y. et al: "Orexins, orexinergic hypothalamic peptides, interact with automatic, neuroendocrire and neuroregulatory systems." Proc Natl.Acad.Sci.USA.96. 748-753 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueta,Y. et al.: "Adrenomedullin-like immunoreactivity in the rat hypothalamo-neurohypophysial tract." Peptides. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto,Y. et al.: "Down regulation of the prepo-orexin gene expression in genetically obese mice." Molecular Brain Research. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nomura,M. et al.: "Effects of centrally odministered pituitary adenylate cyclase-activating polypeptideon c-fos gene expression and heteronuclear RNA for vasopressin in rat paraventricular and supraoptic nuclei." Neuroendocrinology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Serino, R. et al.: "Centrally odministered adrenomedullin increase plasma oyrocin level with induction of c-fos mRNA in the paraventricular and supraoptic nuclei of rat" Endocrinology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Date,Y. et al.: "Enterochromaffin-like cells, a cellular source of uroguanylin rat stomach." Endocrinology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Shibuya,I. et al.: "The Adrenal Chromaffin cell." Hokkaido Univ.Press.Sapporo, 13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山下 博,他: "脳と循環-血圧は脳によって調節される." 共立出版,東京, (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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