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The research on formation of the cell membrane and establishment of the primary metabolism in the primitive earth environments

Research Project

Project/Area Number 10304045
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 地球化学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MURAE Tatsushi  Faculty of Sciences, Kyushu University Prof., 大学院・理学研究院, 教授 (50011747)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Noriaki  Faculty of Sciences, Kyushu University Ass. Prof., 大学院・理学研究院, 助教授 (30242259)
KITAJIMA Fumio  Faculty of Sciences, Kyushu University Assist., 大学院・理学研究院, 助手 (40274427)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥36,470,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥870,000)
Fiscal Year 2001: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 1998: ¥15,000,000 (Direct Cost: ¥15,000,000)
Keywordsprimitive Earth environment / archaea / membrane lipid / cell membrain / biosynthesis / electrospray mass spectrometry / lysine / 化学進化 / エーテル脂質 / Thermplasma acidophilum / Sulfolobus sp. / 膜透過性 / 古細胞 / Thermoplasma acidophilum / Sulfolovus sp. / 有機化合物の膜透過性
Research Abstract

Distribution pattern of carbonaceous matter in Allende carbonaceous chondrite was examined in the imaging by the micro laser Raman spectroscopy in order to know present state of carbonaceous matter in the primitive Earth environment, and it was clarified that the difference existed for structure and present state of the carbonaceous matter between rim and matrix.
The reaction of amino acid by the shock wave was examined in order to clarify the chemical evolution in primitive Earth to indicate the followings : 1) Though the carbonization of the particle surface progresses, when shock wave is applied to the solid particle of glycine, the reaction does not occur inside the solid particle of glycine. (2) It was clarified that the polymerization reaction progressed, when the shock wave was applied to freezing aqueous solution of glycine.
In order to clarify the behavior of cell membrane analogs in the primitive Earth environments, the behavior of the amino acid in porous nylon sphere whose ho … More lls were covered with s film of phosphatidylcholine was examined, and it was clarified that the membrane permeability is affeted by the type of amino acid and the difference of the ionic state of the inside.
The technique using the electro spray ionization mass spectrometry was developed as the convenient technique which analyzed the structure of the membrane lipid of the archaea in order to know the property of the cell membrane in primitive Earth.
The correlation between carbon dioxide concentration and chemical structure of the membrane lipid which the archaea produced on cultivation at high carbon dioxide concentration which imitated the primitive Earth environment was examined. Though the carbon dioxide concentration did not influence in the number of the five-member rings in the isoprenoid chain, it was clarified that the condition of the polar group was affected.
The lysine whose specific position was labeled with deuterium was administered to the halophilic archaea. The uptake pathway of the lysine in biosynthetic pathway of membrane lipid of the halophilic archaea was clarified.
The distribution of the archaea lipid in the sediment in the Kirishima hot spring area was examined in order to develop the means of environment estimation of the primitive Earth, and the paleotemperature was estimated. Less

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] T.Murae: "Fluorescent organic matter in carbonaceous chondrites"Adv. Space Res.. 24・4. 469-476 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Yamauchi: "Practical synthesis of [3-(2H3)mevalonolactone and incorporation experiment of [3-(2H3)methyl]mevalonolactone and [13C]labeled acetate in the biosynthesis of isoprenoidal diether lipids of Halophilic archaea"Bull. Dhem. Soc. Jpn. 73・11. 2513-2519 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Ymauchi: "Obsevation of the pathway from lysine to the isoprenoidal lipid of halophilic archaea, Hlobacterium halobium and Natrinema pallidum, using regiospecifically deuterated lysine"Bull. Chem. Soc. Jpn. 74・11. 2199-2205 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Murae: "Examination of intact ether lipid components of thermoacidophile Sulfolobus sp. by ESI/TOF mass spectrometry"J. Mass Specrom. Soc. Jpn. 49・5. 195-200 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] F.Kitajima: "Evaluating the thermal metamorphism of CM chondrites by using the pyrolytic behanior of carbonaceous macromolecular matter"Geochim. Cosmochim. Acta. 66・1. 163-172 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Murae: "Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry"J. Mass Spectrom.. 37・2. 209-215 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Murae: "Fluorescent organic matter in carbonaceous chondrites"Adv. Space Res.,. 14. 469-476 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Yamauchi, Y. Natsubori, and T. Murae,: "Practical Synthesis of [3-(2H3)methyl]-Mevalonolactone and Incorporation Experiment of [3-(2H3)methyl]Mevalonolactone and [13C]Labeled Acetate in the Biosynthesis of Isoprenoidal Diether Lipids of Halophilic Archaea"Bull. Chem. Soc. Jpn.. 73. 2513-2519 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Yamauchi, S. Endoh, K. Kato, and T. Murae: "Observation of the pathway from lysine to the isoprenoidal lipid of halophilic archaea, Halobacterium halobium and Natrinema palladium, using regiospecifically deuterated lysine"Bull. Chem. Soc. Jpn.. 74. 2199-2005 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Murae, R. Muraoka, and F. Kitajima,: "Examination of intact ether lipid components of thermoacidophile Sulfolobus sp. By ESI/TOF mass spectrometry"J. Mass Spectrom. Soc. Jpn.. 49. 195-200 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] F. Kitajima, T. Nakamura, N. Takaoka, and T. Murae: "Evaluating the thermal metamorphism of CM chondrites by using the pyrolytic behavior of carbonaceous macromolecular matter"Geochim. Cosmochim. Acta. 66. 163-172 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Murae, Y. Takamatsu, R. Muraoka, S. Endoh, and N. Yamauchi: "Facile distinction of neutral and acidic tetraether lipids in Archaea membrane by halogen atom adductions in electrospray ionization mass spectrometry"J. Mass Spectrom.. 37. 209-215 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Murae: "Fluorescent organic matter in carbonaceous chondrites"Adv.Space Res.. 24・4. 469-476 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Yamauchi: "Practical synthesis of [3-(2H3)mevalonolactone and incorporation experiment of [3-(2H3)methyl]mevalonolactone and [13C]labeld acetate in the biosynthesis of isoprenoidal diether lipids of Halophilic archaea"Bull.Dhem.Soc.Jpn. 73・11. 2513-2519 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.yamauchi: "Observation of the pathway from lysine to the isoprenoidal lipid of halophilic archaea, Hlobacterium halobium and Natrinema pallidum, using regiospecifically deuterated lvsine"Bull.Chem.Soc.Jpn. 74・11. 2199-2205 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Murae: "Examination of intact ether lipid components of thermoacidophile Sulfolobus sp.by ESI/TOF mass spectrometry"J.Mass Specrom.Soc.Jpn. 49・5. 195-200 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] F.kitajima: "Evaluating the thermal metamorphism of CM chondrites by using the pyrolytic behanior of carbonaceous macuomolecular matter"Geochim.Cosmochim.Acta. 66・1. 163-172 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Murae: "Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry"J.Mass Spectrom. 37・2. 209-215 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamanaka et al.: "Isotopic fractionation of sulfurin microzones of tidal flat sediments"Gochemical J.. 33. 89-99 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamanaka et al.: "A currently forming petroleum associated with hydrothermal mineralization in a submarine caldera. Kagoshima Bay. Japan"Gochemical J.. 33. 355-367 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murae: "Fluorescent organic matter in carbonaceous chondrites"Adv.Space Res.. 14. 469-476 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamauchi et al.: "Practical synthesis of [3-(^2H_3) methyl] mevalonoiactone and incorpiration experiment of [3-(^2H_3) methyl] mevalonoiactone and [^<13>C] labeled acetate in the biosynthesis of isoprenoial diether lipids of halophilic archaea"Bull.Chem.Soc.Jpn. 73. 2513-2519 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murae et al.: "The chemistry of formaldehyde in the magma ocean system"Proc.32nd.ISAS Lunar Planet.Symposium. 184-187 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Yamanaka: "A currently forming petroleum associated with hydrothermal mimeraligation in a snbmarime caldera,Kagoshime Bay,Japan"Geoohemical Journal. 33. 355-367 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Murae: "Fluorescent organic matter in carbonaeeons chomdrites"Adv.Space Res.. 24. 469-476 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Yamanaka: "Isgtogce fractimation of sulfur in micro gones of tidal flet sediments"Geochemical Journal. 33. 89-99 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Murae: "Fluorescent organic matter in carbonaceous chondrites" Advances in Space Research. 21(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Yamanaka: "Isotopic fractionation of sulfur in micro zones of tidal flat sediments" Geochemical Journal. 33(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Yamauchi: "Solvent-dependent stereochemical anomaly in the nucleophilic addition of ester-derived enolate to bicyclo[3,3,0]octan-2-one system of diacetone glucos-3-ulose" Chem.Lett.475-476 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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