• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mous Saturation mutagenesis project:Construction of sperm bank for chromosome deletion mutants and development of screening system for the mutations

Research Project

Project/Area Number 10358019
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory animal science
Research InstitutionNatiomal Institute of Genetics

Principal Investigator

SHIROISHI Toshihiko  Natiomal Institute of Genetics, Mammalian Genet Lab, Prof, 系統生物研究センター, 教授 (90171058)

Co-Investigator(Kenkyū-buntansha) KOIDE Tsuyoshi  Natiomal Institute of Genetics,Mammalian Genet Lab,Research Associate, 系統生物研究センター, 助手 (20221955)
若菜 茂晴  財団法人 実験動物中央研究所, 研究プロジェクト推進センター, 室長代理 (90192434)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥41,000,000 (Direct Cost: ¥39,200,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2001: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2000: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1999: ¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 1998: ¥13,500,000 (Direct Cost: ¥13,500,000)
KeywordsChromosome deletion / Japanese wild mouse / chlorambucil / Rapid blood clotting / 精子凍結 / 低血糖傾向 / RLGSマッピング / マイクロサテライトマーカー
Research Abstract

We carried out in-depth phenotype characterization of mouse mutants identified in screening for chlorambucil (CHL) treated Gl mice. The mutants that we characterized were ; strain ID numbers, CHL0106 and CHL0225. The former mutant showed lower glucose level in serum, and the latter showed rapid blood clotting. Inheritance of these two mutants was confirmed by cross of the mutants with wild-type mice. For the CHL0225, we analyzed platelet coagulation and counted the number of the platelets in order to examine abnormalities of the function and the amount of the platelets. In addition, we assayed activated thromboplastin time, prothrombin time. Moreover, Amount of fibrimonomer complex antithromobin-thromobin complex was measured in order to examine the function of anti-coagulation factors. As a result, we could not find abnormalities in the all parameters of the CHL0225. It indicated that the mutant CHL0225 is not affected in the known coagulation factors, the anti-coagulation factors and the platelet function, but rather unknown gene in the blood clotting cascade is mutated.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Tanooka, H., Sasaki, H., Shiroishi, T., Moriwaki, K.: "p53 pseudogene dating : identification of the origin laboratory mice"Gene. 270. 153-159 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kikkawa, Y., Miura, I., Takahama, S., Wakana, S., Yamazaki, Y., Moriwaki, K., Shiroishi, T., Yonekawa, H.: "Microsatellite Database for MSM/Ms and JF1/Ms, molossinusu-derived strains"Mammmal. Genome. 12. 750-752 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanooka, H., Sasaki, H., Shiroishi, T. and Moriwaki: "p53 pseudogene dating : identification of the origin laboratory mice"Gene. 270. 153-159 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kikkawa, Y., Miura, I., Takahama, S., Wakana, S., Yamazaki, Y., Moriwaki, K., Shiroishi, T. and Yonekawa H: "Microsatellite Database for MSM"Ms and JF1/Ms, molossinusu-denved strains Mammal. Genome. 12. 750-752 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanooka, H., Sasaki, H., Shiroishi, T, Moriwaki., K: "p53 pseudogene dating : identification of the origin laboratory mice"Gene. 270. 153-159 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kikkawa, Y., Miura, I., Takahama, S., Wakana, S., Yamazaki, Y., Moriwaki, K., Shiroishi, T., Yonekawa H.: "Microsatellite Database for MSM/Ms and JF1/Ms, molossinusu-derived strains"Mammal. Genome. 12. 750-752 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saeki,N. et al.: "Gasdermin (Gs) localizing to mouse chromosome 11 is predominantly expressed in upper gastrointestinal tract but significantly suppressed in human gastric cancer cells."Mammal.Genome. 11. 718-724 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyoshi N. et al.: "Identification of an imprinted gene, Meg3/Gtl2 and its human homologue MEG3, first mapped on mouse distal chromosome 12 and human chromosome 14q."Genes Cells. 5. 211-220 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Watanabe,T. et al.: "Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles."Hum.Mol.Genet.. 9. 3029-3035 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shimada Y. et al.: "adiation-associated loss of heterozygosity at the Znfn1a1(Ikaros) locus on chromosome11 in murine thymic lymphomas."Radiat.Res.. 154. 293-300 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato, H. et al.: "The Genomic organization of type I keratin genes in mice"Genomics,. 56. 303-309 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Komine, Y. et al.: "A novel type of non-coding RNA expressed in the rat brain"Mol. Brain Res.. 66. 1-13 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Moriwaki, K. et al.: "Multiple genes governing biological functions in the genetic backgrounds of laboratory mice and Asian wild mice"Prog. Exp. Tumor Res. Basel, Karger. 35. 1-12 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shum, A.S. et al.: "Retinoic acid induces down-regulation of Wnt-3a, apoptosis and diversion of tail bud cells to a neural fate in the mouse embryo"Mech. Dev.. 84. 17-30 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimizu, K. et al.: "The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein"FEBS letter 456: 295-300,1999. 456. 295-300 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ueda, Y. et al.: "Nucleotide sequences of the mouse globin beta cDNAs in a wild derived new haplotype Hbbwl"Mammal. Genome. 10. 879-882 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hattori, M. et al.: "Cutting edge : homelogous recombination of the MHC class I K region defines new MHC-Linked diabetogenic susceptibility gene(s) in nonobese diabetic mice"J. Immunol. 163. 1721-1724 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tani,M.et al.: "Genomic structure and chromosomal localization of the mouse Oggl gene that is involved in the repair of 8-hydroxyguanine in DNA damage" Genome. 9. 32-37 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hasimoto,Y et al.: "Expression of the Elm1 gene,a novel gene of CCN family,suppresses In vivo tumor growth and metastasis of K-1735 murine melanoma cells" J.Exp.Med.187. 289-296 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nagase,H.et al.: "Novel mutant mice secreting soluble CD4 without expression of membranebound CD4." Eur.J.Immunol.2. 403-412 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi