Grant-in-Aid for Scientific Research (B).
Applied animal science
|Research Institution||Meiji University|
HINO Tsuneo Meiji University School of Agriculture Professor, 農学部, 教授 (50012050)
鷲尾 雄一郎 伊藤忠飼料(株), 総合技術研究部, 研究員
田島 清 農林水産先端技術研究所, 研究第2部, 研究職研究員
長峰 孝文 農林水産先端技術研究所, 研究第2部, 研究職主任研究員
竹中 昭雄 農林水産省, 東北農業試験場, 農林水産技官
ITABASHI Hisao Tokyo University of Agriculture and Technology School of Agriculture Professor, 農学部, 教授 (00280991)
NAGAMINE Takahumi Stuff Institute Animal Section Chief researcher
TAKENAKA Akio Ministry of agriculture and Fishely Tohoku Institute of Agricultural Technical officer
WASHIO Yuichiro Itochu Feed Corp. Research Institute Researcher
TAJIMA Kiyoshi Stuff Institute Animal Section Researcher
|Project Fiscal Year
1998 – 2000
Completed(Fiscal Year 2000)
|Budget Amount *help
¥14,500,000 (Direct Cost : ¥14,500,000)
Fiscal Year 2000 : ¥2,600,000 (Direct Cost : ¥2,600,000)
Fiscal Year 1999 : ¥5,200,000 (Direct Cost : ¥5,200,000)
Fiscal Year 1998 : ¥6,700,000 (Direct Cost : ¥6,700,000)
|Keywords||Rumen / Fumarate reduction / Nitrate reduction / Methanogenesis / Methane inhibitor / cyclodextrin-conjugated halogens / Host-vector system / ルーメン / フマル酸還元 / 硝酸還元 / メタン生成 / メタン抑制物質 / サイクロデキストリン包接化合物 / ホスト・ベクター系 / プロピオン酸 / 牛 / フマル酸 / 硝酸塩 / サリノマイシン / メタン菌阻害剤 / ルーメン微生物 / ギ酸 / シュウ酸 / ブロモクロロメタン|
Attempts were made to reduce methanogenesis in the rumen, and the following results were obtained.
(1) Addition of fumarate reduced methanogenesis without depressing fiber digestion in vitro.
Fumarate-reducing bacteria were selected, and factors affecting fumarate reductase activity were clarified.
(2) Genes encoding phosphoenolpyruvate carboxykinase and pyruvate kinase were cloned, and factors regulating the transcription were examined. The results suggested that the fumarate-reducing pathway could be reduced by increasing. methanogenesis
(3) Methanogenesis was decreased by nitrate and nitrite reduction. Nitrate reductase was shown to be induced by nitrate. Addition of electron donors such as lactate and formate stimulated nitrate and nitrite reduction. This suggests that control of fermentation to increase lactate andformate increases nitrate and nitrite reduction, which reduces methanogenesis.
(4) Middle-chain fatty acids, eucalyptus oil, and cyclodextrin-conjugated halogens (CCH) reduced methanogenesis and stimulated propionate production in vitro. (5) Administration of fumarate or CCH to cattle reduced methanogenesis, enhanced propionate production, and enhanced the digestibility of dry matter and NDF.
(6) Host-vector systems for Prevotella ruminicola and Streptococcus bovis were established, which can be used for gene manipulation of the bacteria.