| Project/Area Number |
10470053
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
UEDE Toshimitsu Hokkaido Univ., Inst.Genet.Med., Prof., 遺伝子病制御研究所, 教授 (00160185)
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| Co-Investigator(Kenkyū-buntansha) |
TOSA Noriko Hokkaido Univ., Inst.Immunol.Sci., Inst., 免疫科学研究所, 助手 (20312415)
IWATA Makoto Mitsubishi Kagaku Inst.Life Sci., Director, 生命科学研究所, 主任
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| Project Period (FY) |
1998 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1998: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | steroid / apoptosis / thymocytes / transgenic mice / アネキシンV / 分化 |
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| Research Abstract |
1. We cloned and sequence a DIG-1 gene that specifically expressed by thymocytes after dexamethason(DEX)treatment.
2. DIG-1 gene encodes 7 transmembrane G protein coupled receptor.
3. We have established 3 lines of DIG-1 transgenic mice.
4. In transgenic mice, thymus were significantly smaller than those in control mice. After DEX injection, the apoptotic cells within thymic cortex increased siginificantly as compared to those in control mice. The apoptotic cells were mainly CD4+CD8+ thymocytes.
5. The expression of apoptosis-related proteins, Bcl-2 and BAX were not significantly different from thosse in transgenic mice.
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