• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pathogenicity and drug resistance of enterohemorrhagic

Research Project

Project/Area Number 10470073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionNiigata University

Principal Investigator

YAMAMOTO Tatsuo  Niigata University School of Medicine Professor, 医学部, 教授 (80095843)

Co-Investigator(Kenkyū-buntansha) 脇阪 紀子  新潟大学, 医学部, 教務補佐員
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1999: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1998: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsEnterohemorrhagic Escherichia coli / O157 : H7 / O128 / EAST1 sequence / Adherence / Enterohemolysin / Anisodamine / Drug resistance / ベロ毒素 / O157 / 感染メカニズム / 溶血毒
Research Abstract

・All the enterohemorrhagic Escherichia coli (EHEC) strains possessed the enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1) gene homologues but with two types of mutations. One of the mutation types (type 1) was strongly associated with the large outbreak episodes in 1996 in Japan. In order to detect EHEC O157 : H7 strains with type 1 sequence by PCR, the primer set (SHEAST1a/SHEAST1b) was successfully constructed. The gene homolugue was also found in the Yersinia. pestis IS sequence.
・Out of eleven O157 : H7 outbreaks, only one outbreak revealed infections due to multiple drug-resistant strains which carried an R plasmid. Tetracyclin, streptomycin, and sulfamethoxazole resistance, which was previously described with O157 : H7 strains isolated from a large outbreak as well as sporadic cases in the United States, were also found in Japan with human and bovine isolates. The entire sequences of the drug resiatnce genes were determined.
・Adherent EHEC O157 : H7 cells were enwrapped by elongated cell membrane on cell surface, while enteropathogenic E. coli (EPEC) was enwrapped by the elongated and crowded microvilli on cell surface. These phenomena must reflect distinct infectious mechanisms of EHEC O157 : H7 (toxin producer) and EPEC (non-toxin producer). Enwrapping of EHEC on cell surface by elongated cell membrane may result in effective toxin delivery across the epithelial cells.
・EHEC strains belonging to serotype O128 adhered to epithelial cells in a pattern of chains with a putative novel adherence factor, and produced EHEC-hemolysin to much greater extents than did the other strains.
・EHEC-hemolysin induced the release of interleukin-1β (IL-1β) but not tumor necrosis factor alpha(TNF-α) from human monocyte at subcytocidal concentrations.
Anisodamine suppressed Verotoxin-induced TNF-alpha production via PGE2-dependent mechanism, and decreased the lethality of Verotoxin in mice. Anisodamine could be a potential drug for treatment of HUS.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Yamamoto T. et. al.: "Status of emerging druing resistance in Shiga toxin-producing Escherichia coli in Japan during 1996 : a minireview (in Japanese)"Nippon Rinsho. 56. 260-271 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhang H-M et. al.: "Effect of subinhibitory concentrations of antibiotics on the production of enterohemolisin by enterohemorrhagic Eschericia coli O157 : H7(in Japanese)"J. J. A. Inf. D. 73. 255-256 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 吉澤麻由子 他: "ベロ毒素によるTNF-αの産生誘導とカスパーゼ3の活性化および抗菌剤による抑制効果の検討"新潟医学会雑誌. 114(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T. et. al.: "The sequence of enterohemorrhagic Escherichia coli and Yersinia pestis that are homologous to the enteroaggregative E.Coli heat-stable enterotoxin gene : cross-species transfer in evolution"FEBS Lett.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T. et. al.: "An atypical adherence pattern of enterohemorrhagic Escherichia coli strains belonging to serotypes O128 : H2 and O128 : H12"Acta. Med. Biol.. 48(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taneika I, et. al.: "The enterohemorrhagic Escherichia coli(EHEC)-hemolysin genes of a Shiga toxin 1(Stx1)-and Stx2-producing, serotype O128 Escherichia coli strain with a greatest hemolytic activity"Acta. Med. Biol.. 48(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimizu T. et. al.: "Helicobacter pylori transmission between a boy with duodenal ulcer and his father"Pediatr. Infect. Dis. J.. 18. 655-656 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohara T. et. al.: "Molecular epidemiology of intrafamilial Mycobacterium tuberculosis infections in Niigata, Japan"Acta. Med. Biol.. 73. 255-256 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山本達男 他: "現代医学の基礎 1),感染と生体防御"岩波書店. 248 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T and Wakisaka N.: "Status of emerging during resistance in Shiga toxin-ptoducing Escherichia coli in Japan during 1996 : a minireview (in Japanese)"Nippon Rinsho. 56. 260-271 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhang H-M and Yamamoto T.: "Effect of subinhibitory concentraions of antibiotics on the production of enterohemolisin by enterohemorrhagic Escherichia coli O157 : H7 (in Japanese)"J. J. A. Inf. D.. 73. 255-256 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimizu T, Oguchi S, Yamashiro Y, Segawa O, Ohkura R, Wakisaka N, and Yamamoto T.: "Helicobacter pylori transmission between a boy with duodenal ulcer and his father"Pediatr. Infect. Dis. J.. 18. 655-656 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohara T, Taneike I, Gejyo F, Tsuchiya T, and Yamamoto T.: "Molecular epidemiology of intrafamilial Mycobacterium tuberculosis infections in Niigata, Japan."Acta Med Biol. 47. 169-173 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yosizawa M, Momose T, Fukuhara T, Hayashi M, Hori Y, Morishima K, Zhang H-M, Ohara T, Kojio S, and Yamamoto T.: "TNF-α induction and caspase-3 activation by Verotoxin and effects of antibiotics on the apotosis-related factors (in Japanese)"Niigata Med. J.. 114 : (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T and Taneike I.: "The sequence of enterohemorrhagic Escherichia coli and Yersinia pestis that are homologous to the enteroaggregative E. coli heat-stable enterotoxin gene : cross-species transfer in evolution"FEBS Lett.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T and Taneike I.: "An atypical adherence pattern of enterohemorrhagic Escherichia coli strains belonging to serotypes O128 : H2 and O128 : H12"Acta. Med. Biol.. (in press), 48. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taneike I, Wakisaka-Saito N, Harada Y, Zhang H-M, and Yamamoto T.: "The enterohemorrhagic Escherichia coli (EHEC)-hemolysin genes of a Shiga toxin 1 (Stx1)- and Stx2-producing, serotype O128 Escherichia coli strain with a greatest hemolytic activity"Acta. Med. Biol.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T. et. al: "GENDAIIGAKU NO KISO 1), KANSEN TO SEITAI BOUGYO"IWANAMI SHOTEN. 248 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamamoto T. et. al.: "Status of emerging druing resitance in Shiga toxin-producing Escherichia coli in Japan during 1996 : a minireview (in Japanese)"Nippon Rinsho. 56. 260-271 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zang H-M et al.: "Effect of subinhibitory concentrations of antibiotics on the production of enterohemolisin by enterohemorrhagic Escherichia coli O157:H7 (in Japanese)"J.J.A.Inf.D.. 73. 255-256 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 吉澤麻由子 他: "ベロ毒素によるTNF-αの産生誘導とカスパーゼ3の活性化および抗菌剤による抑制効果の検討"新潟医学会雑誌. 114(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamamoto T. et. al.: "The sequence of enterohemorrhagic Escherichia coli and Yersinia pestis that are homologous to the enteroaggregative E.coli heat-stable enterotoxin gene: cross-species transfer in evolution"FEBS Lett.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamamoto T. et. al.: "An atypical adherence pattern of enterohemorrhagic Escherichia coli strains belonging to serotypes O128:H12 and O128:H12"Acta. Med. biol.. 48(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taneike I. et. al.: "The enterohemorrhagic Escherichia coli (EHEC)-hemolysin genes of a Shiga toxin 1(Stx1)- and Stx2-producing, serotype O128 Escherichia coli strain with a greatest hemolytic activity"Acta. Med. Biol.. 48(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimizu T. et. al.: "Helicobacter pylori transmission between a boy with duodenal ulcer and his father"Pediatr. Infect. Dis. J.. 18. 655-656 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohara T. et. al.: "Molecular epidemiology of intrafamilial Mycobacterium tuberculosis infections in Niigata, Japan."Acta. Med. Biol.. 47. 169-173 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山本達男 他: "現代医学の基礎 1),感染と生体防御"岩波書店. 248 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tatsuo Yamamoto Noriko Wakisaka: "Status of emereing drug resistance in Shiga toxin-producing Escherichia coli in Japan during 1996: a minireview (in Japanese)" Nippon Rinsho. 56・10. 2718-2729 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 張慧敏, 山本達男: "脹管出血性大腸菌O157のenterchemolysin産生に対する抗生物質の影響" 感染症学雑誌. 73・3. 73-75 (1999)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi