NAKASHIMA Hiroshi Keio University, Dept.of Preventive Medicine and Public Health, Instructor, 医学部, 助手 (80217710)
TAKABAYASHI Toru Keio University, Dept.of Preventive Medicine and Public Health, Assistant Professor, 医学部, 専任講師 (30265780)
|Budget Amount *help
¥13,200,000 (Direct Cost : ¥13,200,000)
Fiscal Year 1999 : ¥1,500,000 (Direct Cost : ¥1,500,000)
Fiscal Year 1998 : ¥11,700,000 (Direct Cost : ¥11,700,000)
In this study, we performed genetic testing to the participants of 6 years follow-up study and investigated the significance of genetic factor on evolution of hypertension or atheroscrerosis. One hundred thirty five persons gave consent to genetic testing and 10 polymorphism of 10 genes including beta 3-adrenergic receptor (B3AR) Trp64Arg, uncoupling protein 1 (UCP1) A-3826G, beta 2-adrenergic receptor (B2AR) Gln27Glu, lipoprotein lipase (LPL) Hind III, apolipoprotein AI (ApoA1) Msp1, apolipoprotein B (ApoB) insertion/deletion, apolipoprotein C (ApoC) Sst1, choresteryl ester transfer protein (CETP) Taq1b, methylenetetrahydrofolate reductase (MTHFR) C667T, angiotensinoger (AGT) M235T were examined. Mean age of first study (S1) was 34.0 years old that of second study (S2) was 40.0 years old.
A-3826G polymorphism of UCP1 gene showed borderline significance (P=0.056) in the BMI of S2. We could not see positive result for AGT on blood pressure or LPL, B3AR, UCP1 and B2AR on glucose metabolism. Msp1 polymorphism of ApoA1 gene was adopted in the multupe regression analysis of triglyceride (TG) at S1 and S2. In S1 Sst1 polymorphism of ApoCIII gene was also adopted. Taq1B plymorphism of CETP gene was adopted in LDL cholesterol examination of S1. Finally, we analyzed the data of beta which indicates atheroscrerosis of carotid artery. Taq1B polymorphig of CETP gene showed borderline significance (p=0.052) in S1 study.
We also performed cross-sectional study composed of 70 males whose age was 20 to 25 years old. Trp64Arg polymorphism of B3AR gene may be one of the determinants of fat distribution, Hind III polymorphism of LPL gene showed positive relation with TG or chiromicron. Again, we could not see positive result for AGT on blood pressure,