| Project/Area Number |
10470216
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokushima (1999)
Kyoto University (1998) |
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Principal Investigator |
DOI Toshio The Tokushima University, School of Medicine, Professor, 医学部, 教授 (60183498)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Takashi The Tokushima University, School of Medicine, Instructor, 医学部, 助手 (40210009)
MIZUNO Akira The Tokushima University, Medical school Hospital, Instructor, 医学部・附属病院, 助手 (80219641)
KUWAJIMA Masamichi The Tokushima University, School of Medicine, Associate Professor, 医学部, 助教授 (00205262)
堀内 久徳 京都大学, 医学研究科, 助手 (90291426)
久米 典昭 京都大学, 医学研究科, 助手 (20252455)
横出 正之 京都大学, 医学研究科, 講師 (20252447)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 1999: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1998: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | mesangial cell / transcription factor / glomerulosclerosis / promoter / TGF-β / MSW / 核転送 |
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| Research Abstract |
Glomerulosclerosis is the most important leading to end-stage kidney disease. The mechanism leading to mesangial cell proleferation and phenotypic change is responsible for the subsequent development of glomeruloscierosis. However, the precise mechanism for glomerular injuries is not completely understood. A novel DNA binding protein (MSW) for type IV collagen gene has a multiple functions including large subunit of DNA replication factor C and DNA binding for angiotensinogen and pro-piomelanocortin corticotropin releasing factor. We found that MSW is a transcription factor, which regulates type IV collagen gene and that MSW is a important modulator in a smooth muscle phenotypic change of mesangial cells. The regulation of the gene expression is associated with translocation of MSW from nucleus to cytoplasm. We found that transforming growth factor-β is an important modulator for the translocation of MSW, which eventually regulates the gene expression. These results indicate that MSW protein plays an important role in regulation of smooth muscle phenotype and may contribute to glomerular cell proliferation and the development of glomerulosclerosis.
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