Grant-in-Aid for Scientific Research (B).
|Research Institution||KYOTO UNIVERSITY|
OGAWA Osamu Kyoto Univ.Graduate Sch.of Med., Prof, 医学研究科, 教授 (90260611)
加藤 哲郎 秋田大学, 医学部, 教授 (40004642)
寺井 章人 京都大学, 医学研究科, 講師 (50243019)
筧 善行 京都大学, 医学研究科, 助教授 (20214273)
寺地 敏郎 京都大学, 大学院・医学研究科, 助教授 (50207487)
山田 仁 京都大学, 大学院・医学研究科, 助手
賀本 敏行 京都大学, 医学研究科, 助手 (00281098)
中村 英二郎 京都大学, 医学研究科, 助手 (90293878)
木下 秀文 京都大学, 医学研究科, 助手 (30324635)
KAMOTO Toshiyuki Kyoto Univ.Graduate Sch.of Med., Lecturer
TERAI Akito Kyoto Univ.Graduate Sch.of Med., Asist.Prof
KAKEHI Yoshiyuki Kyoto Univ.Graduate Sch.of Med., Associ.Prof
NAKAMURA Eijirou Kyoto Univ.Graduate Sch.of Med., Lecturer
KINOSHITA Hldefumi Kyoto Univ.Graduate Sch.of Med., Lecturer
|Project Fiscal Year
1998 – 2000
Completed(Fiscal Year 2000)
|Budget Amount *help
¥12,900,000 (Direct Cost : ¥12,900,000)
Fiscal Year 2000 : ¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 1999 : ¥2,800,000 (Direct Cost : ¥2,800,000)
Fiscal Year 1998 : ¥6,900,000 (Direct Cost : ¥6,900,000)
|Keywords||Urological cancer / Bladder cancer / Prostate cancer / Methylation / Epigenetics / 泌尿器科癌 / 膀胱癌 / 前立腺癌 / メチル化 / エピジェネティック / 尿路性器癌 / メテレーション / 遺伝子変異 / エピジェネティックス|
1) Chromosomes 8 and 9 have been considered to play important roles in the development of bladder cancer. By using the clinical samples of bladder cancer, we determined that an important loci exists on the short arm of chromosome 8, and at least 2 locus on chromosome 9 are associated with bladder carcinogenesis. In addition, we identified a candidate tumor suppressor gene on chromosome 9, named DBCCR1 gene, and analyzed the methylation-status of its promoter region in several bladder cancer cell lines. This experiment showed that the site or the degree of methylation in the DBCCR1 gene promoter showed diversity between the cell lines, and the methylation status was not correlated with the level of expression of DBRCC1. The methylation of DBRCC1 gene was identified in 36 of 69 human bladder tumors (52%).
2) Telomerase is an RNA-dependent DNA polymerase, which can add telomeric repeats to the end of the chromosome, and this enzyme has been considered to have an important role in the cellular immortality. This gene is expressed in some stem cells including germ cells and down-regulated in normal somatic cells. In human cancer, however, this gene is re-expressed presumably by a certain epigenetic mechanism. In this study, we proposed that telomerase activity is a useful marker for the prediction of malignant potential of adrenal tumors, and the detection of telomerase activity in urine is a sensitive non-invasive diagnostic tool.
3) To identify the genetic predisposition to the prostate cancer, a molecular epidemiological analysis was conducted using blood DNA samples obtained from Japanese patients with prostate cancer. We clarified that the genetic polymorphisms in vitamin D receptor gene, CYP17 gene and E-cadherin gene are associated with the risk of prostate cancer.