| Project/Area Number |
10480021
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食生活
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| Research Institution | Nara Women's University |
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Principal Investigator |
YOKOIGAWA Kumio Faculty of Human Life and Environment, Nara Women's University, 生活環境学部, 助教授 (60230637)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Hiroyasu Faculty of Human Life and Environment, Nara Women's University, 生活環境学部, 教授 (80026525)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Escherichia coli / O157 / food / verocytotoxin |
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| Research Abstract |
We examined the growth and verocytotoxin productivity of Escherichia coli O157 grown at low temperature in foods. E.coli strains were isolated from human, bovine and foods, and their genomic profiles and the types of verocytotoxin they produced were analyzed. We used several strains different in the genomic profiles and types of verocytotoxin they produced. The growth rate of E.coli O157 was very low below 4℃, but increased with increase of the temperature. At 8℃, the growth rate changed depending on the types of vegetables. E.coli O157 well grew on cucumber and ginger, but slightly grew on green pepper. When beef and cow's milk were inoculated with E.coli O157, the cells well grew on beef. Difference between E.coli O157 and non-pathogenic E.coli was observed when we used cow's milk : E.coli O157 showed higher growth rate than non-pathogenic E.coli. The verocytotoxin productivity was found to change depending on the strains and types of foods in which the cells grew.
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