| Project/Area Number |
10557024
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Experimental pathology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
UEDE Toshimitsu Hokkaido Univ., Inst.Genet.Med., Prof., 遺伝子病制御研究所, 教授 (00160185)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Masahiro Meneki Seibutsu Co.Ltd., Chief., 次長
村上 正晃 北海道大学, 免疫科学研究所, 助手 (00250514)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1998: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | osteopontin / cancer / prognosis / atherosclerosis / 肉芽腫 / アレル / トランスジェニックマウス / iNOS / ELISA / CD44v / β1インテグリン / 細胞接着 / 細胞遊走 / 転移 |
|---|
| Research Abstract |
1. Highly metastatic adenocarcinoma cells tend to secrete osteopontin(OPN). OPN binds to β1 integrin-containing receptors in an autocrine fashion and induce motility of tumor cells. The association of β1 integrins with CD44v on tumor cell surface is essential for OPN-induced tumor cell motility.
2. The new-vessel formation as defined by CD34 positive endothels was significantly increased in OPN and VEGF positive stage I lung adenocarcinoma tissues and corelated very well with poor prognosis of patients.
3. Upon high fat diet feeding, OPN transgenic mice developed considerably severe atherosclerosis as compared to control mice. In OPN transgenic mice, macrophages within atherosclerotic lesions expressed significant levels of OPN.
4. Upon intravenous injection of zymosan, mice develoved liver granuloma, the development of granuloma was significantly inhibited by simultaneous injection of anti-OPN antibody, indicating the critical role of OPN in the granuloma development.
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