| Project/Area Number |
10557054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Gastroenterology
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| Research Institution | Kanazawa University |
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Principal Investigator |
KANEKO Shuichi Kanazawa University, Medicine Associate Professor, 医学部, 助教授 (60185923)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASAKA Ikuo Sanwa Kagaku Institute, Co., Ltd. Chairman, 所長
OGINO Eiji Kanegafuchi Kagaku, Co., Ltd. Investigator, 研究職
KOBAYASHI Kenichi Kanazawa University, Medicine Professor, 医学部, 教授 (70019933)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Chimpanzee / Hepatitis C Virus / Phasmapheresis |
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| Research Abstract |
Hepatitis C virus (HCV) particles in the serum of hepatitis C patients can be separated into the two fractions according to their density, heavy fraction and light fraction. The former is present with the binding immunoglobulin. We have reported that a higher ratio of the light and the heavy fractions corresponds to the better response rate against interferon therapy. The results indicate that removal of the immunoglobulin-binding fraction may help better response against interferon therapy.
Not only present status of HCV in the serum of chronic hepatitis C patients, but in the liver tissues were studied. Different species of HCV were present in the liver, and the quasispecies of HCV was increased with the severity of liver disease as if the liver consits of a compartment. These results are helpful for the understanding of HCV virology and physiology of this plasma-pheresis study.
Thus, we made several columns for the usage of plasmapheresis which remove immunoglobulin in the serum of chronic hepatitis C patients. The absorption rate with appropriate flow and column volumes was studied by a batch test and a mini-column apparatus, and the best column reagents was chosen.
Two chimpanzees infected with HCV were plasmapheresed using the best reagents in an animal facility. The procedure was finished with safe in terms of circulation, respiration, renal function. Biochemical changes were transient, and there were no infection after the procedure. However, due to anticoagulant used, bleeding at the site of catheterization was found. Serial changes of HCV in the serum and liver tissue were demonstrated in the chimpanzees.
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