Grant-in-Aid for Scientific Research (B)
|Research Institution||Tohoku University|
FUKUSHIMA Kouhei Tohoku University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20271900)
佐々木 一幸 日清製粉, 創薬研究所, 研究員
笹野 公伸 東北大学, 大学院・医学系研究科, 教授 (50187142)
FUNAYAMA Yuji Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (50192315)
NAITO Hiroo Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (90180223)
SASAKI Iwao Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (60125557)
|Project Fiscal Year
1998 – 2000
Completed(Fiscal Year 2000)
|Budget Amount *help
¥7,900,000 (Direct Cost : ¥7,900,000)
Fiscal Year 2000 : ¥1,300,000 (Direct Cost : ¥1,300,000)
Fiscal Year 1999 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1998 : ¥5,000,000 (Direct Cost : ¥5,000,000)
|Keywords||Short boroel Syndrome / total colectomy / molecular biology / epithelial cell / Cell pretiferation / 短腸症候群 / 大腸全摘術 / 分子生物学 / 上皮細胞 / 細胞増殖 / 短腸症侯群 / 細胞分化 / 増殖|
(1)Epithelial gene screening by differential display
a.Ileojeiunal transposition (IJT) model
Ileojejunal transposition was performed using SD rats and samall intestinal epithelial cells were isolated. We subcloned 8 gene and sequenced, resulting that all these clones encoded mitochondrial genes. Differential expresssion was not confirmed, indicating that selection of mitochondrial genes may be due to technical issues accompanied with differential display.
b.Short bowel model
Shor bowel model was established in SD rats and samall intestinal epithelial cells were isolated. We subcloned 11 genes including mitochondrial genes and solute carrier family 9, isoform 3 regulator 1from 80 differential displays.
(2)ornithine decarboxylase(ODC) induction in vitro
IEC6 cells were stimulated 0, 25, 50, and 100 ng/ml glucagon-like peptide 2 (GLP2) and expression of ODC mRNA was examined. Expression of ODC mRNA was stabe after stimulation. In contast, epidermal growth factor, used as a positive control, induced ODC mRNA.
(3)Intestinal adaptation following total colectomy
Rat total volectomy model was established and gene expression of remnant ileal epithelia was analyzed. 11b-hydroxysteroi dehydrogenase type2 (11b-HSD2) was remarkablly induced in the distal ileum probablly due to enhanced aldosterone in blood.
Difficulties in this project was due to 1.inconsistent results from ileojejunal transposition model and 2.limitation of gene screening technique, differential display.