ESTABLISHMENT OF MODELS OF PRECANCEROUS OR CANCEROUS CELLS BY ONCOGENIC TRANSFORMATION OF NORMAL KERATINOCYTES FROM NORMAL HUMAN ORAL CAVITIES
| Project/Area Number | 10557186 |
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Surgical dentistry
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| Research Institution | TOKYO MEDICALAND DENTAL UNIVERSITY |
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Principal Investigator |
SAKAI Eiki Graduate School, Tokyo Medical and Dental University, Research Associate, 大学院・医歯学総合研究科, 助手 (60292976)
上田 晶義(1998) 医科歯科大, 歯学部, 助手 (00272609)
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| Co-Investigator(Kenkyū-buntansha) |
平野 泰正 東京医科歯科大学, 歯学部, 助手
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed(Fiscal Year 2000)
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| Budget Amount *help |
¥12,400,000 (Direct Cost : ¥12,400,000)
Fiscal Year 2000 : ¥1,800,000 (Direct Cost : ¥1,800,000)
Fiscal Year 1999 : ¥3,400,000 (Direct Cost : ¥3,400,000)
Fiscal Year 1998 : ¥7,200,000 (Direct Cost : ¥7,200,000)
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| Keywords | ORAL CANCER / TELOMERASE / N0RAMAL HUMAN KERATINOCYTE / 口腔粘膜上皮角化培養細胞 / テロメテーゼ / ヒト口腔粘膜由来初代培養細胞 / 変異型p53癌抑制遺伝子 / H-ras / SV40 large T / TERT / 口腔粘膜 / ヒト正常粘膜角化細胞 / p53癌抑制遺伝子変異 / アデノウイルスベクター / mRNAサプトラクション法 |
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| Research Abstract |
We studied these themes shown below.
(1) Expression of telomerase activity between cell lines established from human oral squamous cell carcinomas and primary cultured keratinocytes from noraml oral cavities
(2) Expression of telomerase activity between oral squamous cell carcinoma tissues, leukoplakias and normal mucosa from oral cavities
We detected high telomerase activity in all cell lines though 2cell lines showed statistically lower activity than others. But the reasons or the genetical difference for example, expression level of protein of p53, p16 and EGFR still remain unclear. In 6 cell lines, we reported previously mutation of p53 or H-ras, we found that both pathway of p53 and Rb were disrupted samely as shown by Winberg et al.
We detected telomerase activity in 77% of oral squamous cell carcinoma tissues and 47% of leukoplakias while all normal tissues showed no telomerase activity. The activity of the carcinoma tissues showed higher than these of leukoplakias. Furthermore, we detected telomerase activity in 47% of leukoplakias, in spite of almost all lekoplakias we analized showed slight dysplasia. It might suggests that telomerase is activated and cells itself start to gain the ability of immortalization even in the early stage of precancer.
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Report
(4results)
Research Products
(6results)
