| Project/Area Number |
10558102
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Functional biochemistry
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| Research Institution | TOKYO MEDICAL & DENTAL UNIVERSITY |
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Principal Investigator |
HAGIWARA Masatoshi MEDICAL RESEARCH INSTITUTE, TOKYO MEDICAL & DENTAL UNIVERSITY, PROFESSOR, 難治疾患研究所, 教授 (10208423)
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| Co-Investigator(Kenkyū-buntansha) |
MURO Yoshinao NAGOYAMEDICAL UNIVERSITY-ASSISTANT PROFESSOR, 医学部, 助教授 (80270990)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1999: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1998: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | alternative splicing / GFP / arginine methylation / RNAi / SRPK / phosphorylation of SR proteins / カルシトニン / alternative splicing / RT-PCR / SF2 / ASF / リン酸化反応 / スプライシング |
|---|
| Research Abstract |
We tried to establish a new method to screen RNA splicing factors using a calcitonin/CGRP mini gene with GFP expression vector. Although we had expected that regulators for neuron-specific alternative splicing could be cloned with this system, no change of GFP expression was observed in any cells used for this experiment. However, we succeeded to get clones of potential RNA-binding proteins with another method which we originally developed.
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