| Project/Area Number |
10558140
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
KAJIYA Fumihiko Medical Engineering, Kawasaki Medical School, Professor, 医学部, 教授 (70029114)
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| Co-Investigator(Kenkyū-buntansha) |
YADA Toyotaka Medical Engineering, Kawasaki Medical School, Assistant Professor, 医学部, 講師 (00210279)
OGASAWARA Yasuo Medical Engineering, Kawasaki Medical School, Associate Professor, 医学部, 助教授 (10152365)
TSUJIOKA Katsuhiko Medical Engineering, Kawasaki Medical School, Professor, 医学部, 教授 (30163801)
YAMAMORI Shinji R & D Center, Nihon Kohkan Co., Manager, R&Dセンター, 課長
MATSUMOTO Takeshi Medical Engineering, Kawasaki College of Allied Health Professions, Assistant Professor, 臨床工学科, 助教授 (30249560)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 1999: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1998: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | inta-vital needle microscope / NADH / metabolism imaging / myocardial ischaemia / partial oxygen pressure / 代謝イメージング / 代償イメージング |
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| Research Abstract |
We made in-vivo analysis on images of metabolic function and oxygen partial pressures in endocardial microcirculation where is the most frequent site of myocardial ischaemia. The purpose of the present study was to elucidate coronary microcirculation pathophysiologically. We achieved ;
1) development of needle lens
2) development of an NADH excitor and its fluorescence observer
(excitation at 370 nm, fluorescence at 460 mm)
3) development of Pd-porphine excitor and its fluorescence observer
(excitation at 545 nm, fluorescence at 610 nm)
4) development of micro-circulation image observer
(wave length : 545 nm)
With these developments, we confirmed that myocardial metabolism imaging was made possible.
After evaluating the performance of the developed intra-vital needle microscope, we firstly applied this system to porcine and canine experiments. Obtaining metabolism images by NADH fluorescence, capillary blood flow distribution of digital radiogram was measured by perfusing molecular blood flow tracers, which specifically bind to α2 receptors of capillary endothelial cells.
Secondly, we performed metabolism imaging at the region of endocardial microcirculation under the conditions of control and myocardial ischaemia. We compared NADH fluorescence intensity, coronary micro-vascular diameters and blood flow distribution under the control condition of 100 mmHg perfusion pressure and under the ischaemic condition of low perfusion pressure at 40 and 60 mmHg. There was heterogeneity with patchy pattern in NADH fluorescence distribution during reperfusion and hypoxic perfusion.. The sizes of patchy area in both images are about several hundred microns. In summary, there is a significant correlation between NADH fluorescence and blood flow distribution, indicating the similarity between metabolic function distribution and blood flow distribution.
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