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The regulatory mechanism of phototransduction pathway in rhabdomeric photoreceptors.

Research Project

Project/Area Number 10640672
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHyogo College of Medicine

Principal Investigator

SUZUKI Tatsuo  Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90068544)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCell signaling / Photoreceptor / Phototransduction / Rhodopsin / GTP-binding protein / Phospholipase C / Proteinkinase C / ラプドーム
Research Abstract

1. Reconstitution system of rhodopsin/G protein/phospholipase C was established, and activation of phospholipase C was observed in the presence of GTP and light. It was experimentally confirmed in vitro that the rhodopsin/G protein/phospholipase C system is able to work as the major reaction pathway of phototransduction.
2. Under the light conditions, translocation of G protein from membrane to cytosol was found in two species, Procambarus and Octopus. The cytosolic type was an inactive form of G protein and it did not couple with rhodopsin. It was suggested that a fatty acid is attached to the membrane-bound form and unattached to the cytosolic form.
3. The C-terminal peptide of phospholipase C was cleaved by a calcium-dependent protease, and the truncated phospholipase C was not activated by G protein. It was directoly proved in reconstitution system that the C-terminal peptide is the regulatory domain of the enzyme.
4. cDNA of phospholipase C was cloned in Watasenia, which encoded a protein of M.W.125,670 (1, 112 amino acids). The protease-cleavage site was determined by mass spectrometry and amino acid sequencing to be N-terminal side of G(860), A(861) and K(863).
5. Proteinkinase C, which is activated by diacylglycerol and calcium, was isolated from squid retina. It was found that phospholipase C was efficiently phosphorylated by the purified proteinkinase C.
6. The results of this study suggested that the translocation of G protein and the limited proteolysis of phospholipase C are candidates of regulatory mechanism of phototransduction in rabdomeric photoreceptors.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Terakita Akihisa: "Light-regulated localization of the beta-subunit of Gq-type G-protein in the crayfish photoreceptors."Journal of Comparative Physiology A. 183. 411-417 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki Tatsuo: "Regulation of squid visual phospholipase C by activated G-protein alpha."Comparative Biochemistry and Physiology A.. 122. 369-374 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Narita Kinya: "Structural and functional differences of two forms of GTP-binding protein, Gq, in the cephalopod retina."Comparative Biochemistry and Physiology B.. 123. 319-327 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Terakita, Akihisa: "Light-regulated localization of the beta-subunit of Gq-type G-protein in the crayfish photoreceptors."J.Comp.Physiol.A. 183. 411-417 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki, Tatsuo: "Regulation of squid visual phospholipase C by activated G-protein alpha."Comp.Biochem.Physiol.A. 122. 369-374 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Narita, Kinya: "Structural and functional differences of two forms of GTP-binding protein, Gq, in the cephalopod retina."Comp.Biochem.Physiol.B. 123. 319-327 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Terakita Akihisa: "Light-regulated localization of the beta-subunit of Gq-type G-protein in the crayfish photoreceptors."Journal of Comparative Physiology A. 183. 411-417 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki Tatsuo: "Regulation of squid visual phospholipase C by activated G-protein alpha."Comparative Biochemistry and Physiology A.. 122. 369-374 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Narita Kinya: "Structural and functional differences of two forms of GTP-binding protein, Gq, in the cephalopod retina."Comparative Biochemistry and Physiology B.. 123. 319-327 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tatsuo Suzuki: "Regulation of squid visual phospholipase C by activated G-protein alpha"Comparative Biochemistry and Physiology A. 122. 369-374 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kinya Narita: "Structural and functional differences of two forms of GTP-binding protein,Gq,in the cephalopod retina."Comparative Biochemistry and Physiology B. 123. 319-327 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akihisa Terakita: "Light-regulated localization of the beta-subunit of Gq-type G-protein in the crayfish photoreceptors" Journal of Comparative Physiology A. 183. 411-417 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tatsuo Suzuki: "Regulation of squid visual phospholipase C by activated Gprotein-alpha" Comparative Biochemistry and Physiology A. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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