| Project/Area Number |
10650760
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
反応・分離工学
|
|---|
| Research Institution | KYOTO INSTITUTE OF TECHNOLOGY |
|---|
Principal Investigator |
YOSHIKAWA Masakazu Dep. of Polym. Sci. and Eng. Kyoto Institute of Technology, Professor, 繊維学部, 教授 (60158417)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Chiral Recognition / Molecular Recognition / Optical Resolution / Molecular lmprinting / Amino Acids / Peptides / Membrane Separation / Permselectivity |
|---|
| Research Abstract |
It is not only an interesting but also an unsolved subject to develop artificial receptors. The molecular imprinting technique is a facile method to impart molecular recognition sites in synthetic polymers. In the present study, eight kinds of tripeptide derivatives, which consists of Glu (OBzl) and Phe, were adopted as candidate materials to form chiral recognition sites.
The membranes imprinted by L-isomer recognize L-isomer in preference to corresponding D-isomer.
To study the substrate specificity of the recognition site, the adsorption isotherms were investigated. The adsorption isotherm for D-isomer is a straight line passing through the origin, implying that the D-isomer is adsorbed by the membrane without any specific interaction. On the other hand, that of L-isomer has a complicated profile, implying that the adsorption consists of non-specific adsorption and adsorption on a specific recognition site for the L-isomer.
By adopting optimum potential difference, electrodialysis of the racemic amino acid showed that permselectivity directly reflects its adsorption selectivity.
|
