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Genetical and molecular biological analyses on seed dormancy of Echinochloa plants

Research Project

Project/Area Number 10660047
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YAMASUE Yuji  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (60093332)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsEchinochloa plants / Seed dormancy / Inheritance of seed dormancy / ATPas / HィイD1+ィエD1-ATP / ヒエ属雑草 / 種子発芽
Research Abstract

(1) Cultivated form of E. oryzicola genetically deficient in seed dormancy was reciprocally hybridized with its weedy form with deep seed dormancy. Frequency distribution of FィイD22ィエD2 hybrids with different dormancy levels in their seeds suggested two major genes for control of the seed dormancy. Seeds derived from inbreeding of all of the FィイD22ィエD2 hybrids that appeared to be recessive homozygotes for the two genes were nondormant.
(2) Two mRNA that specifically expressed in dormant seeds were found at an early stage of imbibition when mRNA were compared between dormant and nondormant seeds of both tetraploid and hexaploid Echinochloa plants by differential display. Complimentary DNA of the one (EcD400) had no homology with known proteins, but that of the other (EcD700) was suggested to be mitochondrial HィイD1+ィエD1-ATPase by a database homology assessment. With results of our previous studies, this finding may indicate that seed dormancy of this genus is maintained by aerobic respiration, and the dormancy is released by increasing activity of anaerobic respiration.
(3) Both the above hybridization experiment and molecular biological analyses suggested that two genes were involved in the mechanism of seed dormancy. But further studies are required to clarify dormancy levels of the advanced progenies of the hybrids and roles of the respiratory enzyme and EcD400 in a seed of Echinochloa plants.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 広末 徹、山末祐二、薮野友三郎: "タイヌビエの栽培型×雑草型F_2雑種における種子脱落性と休眠性について(続報)"雑草研究. 43(別). 68-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Hirosue, Y.Yamosue and T.Yabuno: "Shattering habit and dormancy of spikelets in a cultivated form of E.oryzicola recently found in China"Weed Research. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 深尾 武、井田正三、山末祐二: "ヒエ属雑草の休眠種子に特異的に発現するH^+-ATPase mRNAの単離と解析"雑草研究. 45(別)(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Hirosue, Y. Yamasue and T. Yabuno: "On Seed shattering habit and dormancy of F2 hybrids between cultivated and weedy forms of E. oryzicola."J.Weed Sci. and Technology. 40(supplement). 68-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Hirosue, Y. Yamasue and T. Yabuno: "Shattering habit and dormancy of spikelets in a cultivated form of E. oryzicola recently found in China"Weed Research. (in Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Fukao, S. Ida and Y. Yamasue: "Isolation and analyses of H+ATPase mRNA specifically expressed in dormant seeds of Echinochloa weeds"J.Weed Sci. and Technology. 40(supplement), (in Press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I.Hirosue, Y.Yamasue and T.Yabuno: "Shattering habit and dormancy of spikelets in a cultivated form of E.oryzicola recently found in China"Weed Research. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] 深尾武,井田正三,山末祐二: "ヒエ属雑草の休眠種子に特異的に発現するH^+-APTase mRNAの単離と解析"雑草研究. 45(別)(印刷中). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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