| Project/Area Number |
10660111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Arid Land Research Center, Tottori University |
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Principal Investigator |
SUGIMOTO Yukihiro Arid Land Research Center, Tottori University, Associate Professor, 乾燥地研究センター, 助教授 (10243411)
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| Co-Investigator(Kenkyū-buntansha) |
INANAGA Shinobu Arid Land Research Center, Tottori University, Professor, 乾燥地研究センター, 教授 (40124664)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Menispermum dauricum / root culture / Striga / germination / stimulant / strigol / 根寄生雑草 / Striga hermonthica / 発芽刺激物質 / Menispermum dauricum / Stephnia cepharantha / タマサキツヅラフジ |
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| Research Abstract |
Tissue culture techniques were employed to explore capabilities of several plants to produce Striga germination stimulants. The techniques were used for the first time and offered several advantages over the commonly used conventional techniques of growing plants hydroponically. They were less laborious, required less space, allowed control of the environment and eliminated problems caused by microorganisms. Furthermore, as in the case of several other secondary metabolites from tissue cultures by manipulation of medium composition.
Menispermum dauricum root cultures were the most efficient producer of Striga germination stimulants compared across all plants tested. The cultures produced at least 3 active compounds. High-stimulant-producing tissue cultures were established by manipulation of culture conditions. A modified B5 medium containing 35.7mM nitrogen at a NOィイD23ィエD2ィイD1-ィエD1/NHィイD24ィエD2ィイD1+ィエD1 ratio of 1 : 42, 0.1mM FeィイD12+ィエD1, 1.0 mM CaィイD12+ィエD1, 0.55 mM inorganic phosphorus, 0.28 mM inositol, 4.1 μM nicotinic acid, 3.7 μM pyridoxine hydrochloride, 14.8 μM thiamine hydrochloride, 1 μM NAA, and 4% sucrose sustained root growth for a longer period and increased root biomass by >30% and stimulant production by 5-fold, in comparison to the standard B5 medium supplemented with 3% sucrose and 1 μM NAA.
The major Striga germination stimulant produced by M. dauricum root cultures displayed similar chromatographic behavior, similar UV and mass spectra to authentic strigol. Accordingly the major Striga germination stimulant produced by M. dauricum root cultures was most likely strigol or a strigol-like molecule.
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