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Elucidation of immnoregulating function of casein phosphopeptide

Research Project

Project/Area Number 10660258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionShinshu University

Principal Investigator

OTANI Hajime  Faculty of Agriculture, Shinshu University Professor, 農学部, 教授 (30109201)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsMilk casein / Casein phosphopeptides / Immunoregulator / Cytokines / Intestinal IgA / Mitogenic activity / Oral administration / Lymphocytes / 経口投与試験 / 機能性食品事材 / カゼイン / 免疫賦活 / IgA / 経口投与 / 豚 / 機能性飼料 / 牛乳 / 免疫グロブリン生産 / リンパ球増殖 / マイトージェン活性 / 特定保健用食品
Research Abstract

An immunomodulating peptide was purified from a trypsin digest of bovine αs1-casein and identified as residues 59-79 in bovine αs1-casein. The sequence 59-79 has a Phosphoserine-rich region and is called one of casein phosphopeptides. Bovine β-casein aloso possesses phosphoserin-rich region. Hence, the author isolated β-casein fragment 1-25, one of the casein phosphopeptides, from tryptic β-casein and confirmed that the phosphopeptide displayed the immunomodulating action as well as the casein phosphopeptide from bovine αs1-casein.
The casein phosphopeptides displayed a mitogenic activity and stimulated the mitogen-induced proliferations and the immunoglobulin production via the stimulation of IL-5 and IL-6 produced from Th2 cells in mouse spleen cell cultures. The oral adminstration of casein phosphopeptides enhanced the levels of antigen-specific IgA and total IgA in mice and piglets. Moreover, the immunostimulating activity was confirmed to a phosphopeptide having at least 2 phosphoserine residues, i.e., SerP-Leu-SerP.It has been widely reported that various kinds of casein phosphopeptides are produced from bovine caseins by the action of various kinds of proteolytic enzymes. Thus, the casein phosphopeptides prepared from bovine casein digests are beneficial foodstuffs for the enhancement of mucosal IgA responses in man and livestock.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] I.Hata,S.Higashiyama & H.Otani: "Identification of a phosphopeptide in bovine αs1-casein digest as a factor influencing proliferation and immunogloblin production in lymphocyte cultures"Journal of Dairy Research. 65. 569-578 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 大谷元: "カゼインホスホペプチドとκ-カゼイノグリコペプチドの免疫調節機能"ミルクサイエンス. 47. 183-193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Hata,J.Ueda & H.Otani: "Immunostimulatory action of a commercially available casein phosphopeptide preparation, CPP-III"Milchwissenschaft. 54. 3-7 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Otani,Y.Kihara & M.Park: "The immunoenhancing property of a dietary casein phosphopeptide preparation in mice"Food and Agricultural Immunology. 12. 165-173 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Otani,H.Kitamura et al.,: "Enhancement of intestinal IgA levels in piglets by oral administration of a commercially available casein phosphopeptide preparation"Milchwissenschaft. 55. 429-432 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 木原行啓,大谷元: "殺菌条件の異なる牛乳からカゼインホスホペプチドの調製とそれらのマウス脾臓細胞の増殖に及ぼす影響"ミルクサイエンス. 49. 73-79 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 大谷元: "21世紀の食・環境・健康を考える"共立出版(印刷). 62-74 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hajime Otani: "Current Research of Advanced Series in Agricultural & Biological Chemistry"World Wide Research Network Publisher(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Isao Hata, Shigeki Higashiyama and Hajime Otani: "Identification of a phosphopeptide in bovine αs1-casein digest as a factor influencing proliferation and immunogloblin production in lymphocyte cultures"Journal of Dairy Research. 65. 569-578 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hajime Otani: "Immunomodulating activity of casein phosphopeptides and κ-caseinoglycopeptides, and a conclusion relating to their physiological significance in neonates"Milk Science. 47. 183-193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Isao Hata, Jitsuo Ueda and Hajime Otani: "Immunostimulatory action of a commercially available casein phosphopeptide preparation, CPP-III, in cell cultures"Milchwissenschaft. 54. 3-7 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hajime Otani, Yukihiro Kihara and Minkyu Park: "The immunoenhancing property of a dietary casein phosphopeptide preparation in mice"Food and Agricultural Immunology. 12. 165-173 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hajime Otani, Hiroshi Kitamura, Minkyu Park, Yoshihiro Kihara, Toshio Oshida, Seiji Kusuhara and Kazuhiko Sawada: "Enhancement of intestinal IgA levels in piglets by oral administration of a commercially available casein phosphopeptide preparation"Milchwissenschaft. 55. 429-432 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yukihiro Kihara and Hajime Otani: "Preparation of casein phosphopepetides from different kinds of pasteurized milks and their action on proliferative responses of mouse spleen cells in cell cultures"Milk Science. 49. 73-79 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hajime Otani: "Enhancement of IgA levels by casein phosphopeptides"Current Research of Advanced Series in Agricultural & Biological Chemistry. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Otani,Y.Kihara & M.Park: "The immunoenhancing property of a dietary casein phosphopeptide preparation in mice"Food and Agricultural Immunology. 12. 165-173 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Otani,H.Kitamura, et al.: "Enhancement of intestinal lgA levels in piglets by oral administration of a commercially available casein phosphopeptide preparation"Milchwissenschaft. 55. 429-432 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 木原行啓,大谷元: "殺菌条件の異なる牛乳からカゼインホスホペプチドの調製とそれらのマウス脾臓細胞の増殖に及ぼす影響"ミルクサイエンス. 49. 73-79 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Otani: "Current Research of Advanced Series in Agricultural & Biological Chemistry"World Wide Research Network Publisher (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] H. Otani, Y. Kihara & M. Park: "The immunoenhancing property of a dietary cassin phosphopeptide preparation in mice"Food Agric. Immunol.. 12(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. A. Matin, M. Monnai & H. Otani: "Isolation and characterization of a cytotoxic pentapeptide, k-casecidin, from bovine k-casecin degested with bovine trypsin"Anim. Sci. J.. 71(2). 197-207 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. A. Matin & H. Otani: "Release of cytotoxic glycopeptides from human acid casein fraction by the action of stomach proteinases"Milchwissenschaft. 55(1). 6-10 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Odashima, F. Takano & H. Otani: "Inhibitory properties of Japanise quail ovoinhibitor toward lymphocyte proliferation"Anim. Sci. J.. 71(1). 92-102 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 大谷 元(唐澤豊編): "21世紀の食・環境を考える"共立出版. 227(13) (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I.HATA, J.UEDA & H.OTANI: "Immunostimulatory action of a commercially available casein phosphopeptide preparation, CPP-III, in celi culture" Milchwissenschaft. 54(1). 3-7 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] I.HATA, S.HIGASHIYAMA & H.OTANI: "Identification of a phosphopeptide in bovine asl-casein digest as a factor influencing proliferation and immunogloblin production in lymphocyte cultures" J.Dairy Res.65(4). 569-578 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.OTANI & G.MIZUMOTO: "Suppresive effect of lysozymes and a-lactalubumins on mitogen-induced proliferative responses of mouse lymphocytes" Anim.Sci.Technol.65(11). 1029-1039 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大谷 元: "病原性微生物に対する抗体を多量に含む生乳の生産と利用" ミルクサイエンス. 47(2). 63-75 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大谷 元: "牛乳カゼイン由来のペプチドの栄養生理機能" ミルクサイエンス. 47(3). 183-193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大谷 元: "牛乳・乳製品のおいしさを左右する風味成分" 日本畜産学会北陸支部会報. 75. 2-8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大谷元(吉川正明他編): "ミルクの先端機能" 弘学出版, 276(24) (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大谷元(鈴木敦士他編): "食品成分シリーズ「タンパク質の科学」" 朝倉書店, 206(20) (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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