|Budget Amount *help
¥1,400,000 (Direct Cost : ¥1,400,000)
Fiscal Year 1999 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1998 : ¥800,000 (Direct Cost : ¥800,000)
This study was undertaken to clarify the localization of pertussis toxin-sensitive G protein in the cilia of tissues, involving the ependymal cells lining the ventricular cavity of nervous system, the tracheal epithelium, the epithelium of the oviduct.
Adults rats were anesthetized and fixed by perfusion through the heart and issues from brains, trachea, and oviducts were dissected out. Sections for light and electron microscopic examination were processed using antibodies against α subunits of Go and Gi subtypes (Gi1, Gi2, Gi3). In addition, the cilia-rich pellet from the ventricular surface of brains were obtained and the richmess of pertussis toxin-sensitive G protein was shown in this pellet compared with the pellet from the motor cortex.
We found Gi2 was exclusively localized, at light microscopic level, in the cilia of ependymal cells, the cilia of tracheal epithelium and oviduct epithelium. Immunoelectron microscopy revealed that Gi2 was found predominantly in the ciliary membrane of these tissues. Immunoblot analysis demonstrated higher levels of Gi2 in the ependymal cilia-rich pellet than in the motor area of the parietal cortex, supporting the Immunohistochemical observations.
There are some reports that when Bordetella pertussis us inoculated in vivo into the cerebral ventricles of mice, the bacteria multiply only around the ciliated ependymal cells, while the infection by bacteria affected only the ciliated respiratory cells of cultured trachea and caused their ciliostasis with nonciliated cells almost intact. These observations and the results of the present study suggest that Gi2 is localized in the ciliary membrane of ependymal cells and ciliated epithelial cells of trachea and oviduct and seems to be involved in the motile function of these ciliated cells.