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Basic Study of Lung Injuries and Neutrophils - Isolation and Purification of Alveolus-Derived Neutrophil Inhibitory Factor and Its Effects on The Development of Lung Injuries -

Research Project

Project/Area Number 10670531
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

SUWABE Akira  DEP. OF MED., YAMAGATA UNIV., ASSOCIATE PROFESSOR, 医学部, 助教授 (20241713)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
KeywordsLUNG SURFACTANT / LUNG INJURY / NEUTROPHILS / SUPEROXIDES / ALVEOLAR TYPE II CELLS
Research Abstract

Pulmonary surfactant and alveolar type II epithelial cells play important roles in lung defense mechanisms. We reported that native surfactant and its components (SP-A and phospholipid liposomes) as well as artificial surfactant (Surfactant-TA, S-TA) inhibited adherence, chemotaxis, release of superoxides and elastase of human peripheral neutrophils. Neutrophils reacted with S-TA showed characteristics of apoptotic cells. We also demonstrated that S-TA inhibited proliferation of rat lung fibroblasts and that S-TA protected a type II cell monolayer and enhanced its surfactant metabolism.
Surfactant replacement therapy significantly decreases a mortality of ARDS patients. Surfactant may improve gas exchanges by preventing alveolar collapse. We showed that superoxide production from neutrophils obtained from the hamster lungs, in which S-TA was instilled 24 hours after bleomycin administration, was significantly inhibited, compared to neutrophils from control animals. The rats, which were replaced with S-TA twenty four hours and seven days after receiving bleomycin administration, showed a decrease in lung collagen content, compared to control animals. These findings suggest that S-TA may inhibit neutrophil activation or fibroblast proliferation and may protect type II cells from their damage.
In summary, pulmonary surfactant was shown to have significant effects on functions of inflammatory cells, such as alveolar macrophages, lymphocytes, or neutrophils, as well as on those of stroma cells, such as fibroblasts or type II cells. Surfactant, together with type II celles, may be involved in the development of some kinds of pulmonary diseases. In the future aspects, development of new kinds of artificical surfactant as a therapeutic tool may be required, along with the studies on clinical usefulness and its mechanisms.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 諏訪部章: "サーファクタント治療"臨床科学. 34. 797-804 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suwabe A.,Otake K.,Yakkuwa N.,Suzuki H.,Ito M.,Tomoike H.,Saito Y.,Takahashi K.: "Artifical Surfactant (Surfactant TA)Modulates Adherence and superoxide production of Neutrophils"Am J Respir Crit Care Med. 158. 1890-1899 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 諏訪部章,伊藤未幸,富永真琴,高橋敬治: "実験肺傷害に対する人工サーファクタント投与の効果―肺線維化と各肺細胞機能に対する影響―"臨床呼吸生理. 30. 11-15 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 諏訪部章: "肺の生体防御―肺サーファクタントと肺胞II型細胞の役割"日本界面医学会雑誌. 30. 1-12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 諏訪部章: "呼吸器関連の細胞株培養と応用・第1回、肺胞上皮細胞(方法論)"分子呼吸器病. 3. 46-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 諏訪部章: "呼吸器関連の細胞株培養と応用・第2回、肺胞上皮細胞(応用研究例)"分子呼吸器病. 3. 112-118 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akira Suwabe: "Therfactant Therapy."Rinsyou Kagaku. 34(6). 797-804 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akira Suwabe: "Artificial Surfactant (Surfactant TA) Modulates Adherence and Superoxide Production of Neutrophils"Am J Respir Crit Care Med. 158. 1890-1899 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akira Suwabe: "Effects of artificical surfactant on the experimental lung injury. - Studies on lung fibrosis and various lung cells -"Clinical Pulmonary Phisiology. 30(2). 11-15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akira Suwabe: "Lung defense mechanisms - Roles of lung surfactant and alveolar type II cells -"J. Jpn.. Med. Soc. Biol. Interface. 30. 1-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akira Suwabe: "Cell culture and its applocations in Respirology (1) : Aleveolar type II cells (Methodology)"Molecular Respirology. 3(1). 46-48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akira Suwabe: "Cell culture and its applocations in Respirology (2) : Aleveolar type II cells (Application to Researches)"Molecular Respirology. 3(2). 112-118 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 諏訪部 章: "肺の生体防御-肺サーファクタントと肺胞II型細胞の役割"日本界面医学会雑誌. 30. 1-12 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 諏訪部 章: "呼吸器関連の細胞株培養と応用・第1回肺胞上皮細胞(方法論)"分子呼吸器病. 3(1). 46-48 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 諏訪部 章: "呼吸器関連の細胞株培養と応用・第2回肺胞上皮細胞(応用研究例)"分子呼吸器病. 3(2). 112-118 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 諏訪部 章: "サーファクタント治療" 臨床科学. 34(6). 797-804 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suwabe A, Otake K, Yakuwa N. Suzuki H, Ito M, Tomoike H, Saito Y, Takahashi K: "Artificial Surfactant (Surfactant TA) modulates Adherence and Superoxide production of Neutrophils" Am J Respir Crit Care Med. 158. 1890-1899 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 諏訪部章,伊藤末幸,冨永真琴,高橋敬治: "実験肺傷害に対する人工サーファクタント投与の効果-肺線維化と各肺細胞機能に対する影響" 臨床呼吸生理. 30(2). 11-15 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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