|Budget Amount *help
¥2,000,000 (Direct Cost : ¥2,000,000)
Fiscal Year 1999 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1998 : ¥1,400,000 (Direct Cost : ¥1,400,000)
The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP ; n=5), nonspecific interstitial pneumonia (NSIP ; n=5), and normal parts of surgically resected lungs (control ; n=5). The results indicated that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7±6.21% ; NSIP, 32.8±5.46 ; controls, 28.5±3.51, p<0.01 in UIP versus NSIP or control) ; (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts ; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not ; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-β_1 by immunoblots, respectively ; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-β_1(fibronectin : UIP 289±47.1 ng/ml, NSIP 121±23.0, control 118±16.0 ; TGF-β_1 : UIP 798±119 pg/ml, NSIP 246±69.1, control 247±53.6, p<0.01 in UIP versus NSIP or control) ; (5)the contractility positively correlated with the amount of either fibronectin (r=0.867, p<0.001, n=15) or TGF-β_1(r=0.939, p<0.001, n=15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and upregulated control fibroblasts.