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The molecular biological analysis for the release mechanism of endothelial-derived vasoactre substances.

Research Project

Project/Area Number 10670631
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

SHIN Wee Soo  Instructor, Health Service Center, 保健管理センター, 助手 (10211971)

Co-Investigator(Kenkyū-buntansha) TOYO-OKA Teruhiko  Professor of Medicine and Director of Health Service Center, 医学部, 教授 (00146151)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsVascular endothelium / nitre oxide / IPィイD23ィエD2 / intracellular calcium ions / antisense aligouucleotides / 血管内皮細胞 / 血管平滑筋細胞 / 細胞内カルシウム / イノシトール3燐酸 / マイクロインジンクション
Research Abstract

1. Anti, IP3 receptor antibody : made from C-terminal of type I IP3 receptor, showing single band on the Western Blotting and immunologically positive for bovine endothelial cells and rat cerebellum (membrane fraction)
2. Effects of anti-IP3 receptor Ab on [CaィイD12+ィエD1]ィイD2iィエD2 ATP-induced CaィイD12+ィエD1 elevation was inhibited by anti IP3 receptor antibody dose-dependently. It inhibited both initial peak and sustained phase of CaィイD12+ィエD1 elevation.
3. Effects of NOS inhibitor l-NMMA : Cotreatment of l-NMMA with anti IP3 receptor antibody is not enough for complete block of IPィイD23ィエD2 induced CaィイD12+ィエD1 release, revealing the autocrine action of NO. These results have shown that this method can analyze each signal transduction step in endothelial cells using intracellular calcium ions as a biological marker and also can apply more precise mechanism of vasoactive substances such as NO or endothelin.
4. Transfection experiments : As a result of transfection of a marker gene (LacZ) into smooth muscle cell line, Lipofection is superior to HVJ-liposome method or to plasmid alone. Currently, antisense ODNs to NOS are transfected into VSMC or EC and [CaィイD12+ィエD1]ィイD2iィエD2 are measured.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Wang Y., Chen J., Wang Y., et al.: "Not type-3, but type-2 inositol trisphosphate (IP_3) receptors is crucial for IP_3 induced Ca^<2+> release and proliferation in vascular smooth muscle cells"Circulation. 100. I209 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang Y., Chen J., Wang Y., et al.: "Down-regulation of type-1 IP_3R and functional IICR in VSMC by basal release of nitric oxide from endothelial cells"Circulation. 100. I623 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawaguchi H., Shin WS., Okai Y., et al.: "Apoptolic cell death in acute myocardial injury induced by isoproterenol is mediated by nitric oxide"J. Cardiovasc. Pharmacol.. 34. S25-S28 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawada T., Shin WS., Nakatsuru Y., et al.: "Precise identification of gene products in hearts after in vivo gene transfection using Sendai virus-coated proteoliposome"Biochem. Biophys. Res. Commun.. 259. 408-413 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawada T., Nakatsuru Y., Sakamoto A., et al.: "Strain- and age-dependent loss of sarcoglycan complex in cardiomyopathic hamster hearts and its re-expression by 8 salcoglycan gene transfer in vivo"FEBS Lett.. 458. 405-408 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki J., Shimamoto R., Nishikawa J., et al.: "Morphological onset and early diagnosis in aperal hypertrophic cardio-myopathy: A long terminalysis with unclear magnetic resonance imaging"J. Am. Cott. Cardiol..

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 申 偉秀、豊岡 照彦: "Ca拮抗薬のすべて(第二版)分担分:Ca拮抗薬の血管平滑筋に対する作用"先端医学社. 9/447 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 申 偉秀: "薬物障害ガイド:分担分:血管*腫"南山堂. 3/ (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang Y, Chen J, Wang Y, et al.: "Not type-3, but type-I inositol tri3 phosphate (IP_3) receptors is Crucial for IP_3 induced Ca^<2+> release and prolifermation in vascular smooth maycledbls cells"Crunlation. 100. I209 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wang Y, Wang Y, Chem J, et al.: "Down-regulation of type-1 IP_3R and functional IZCR in VSMC by basal release of nitrit oxide from enduthelial cells"Cirmlation. 100. I623 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawaguchi H, Shin WS, Okai Y, et al.: "Apoptotic cell death in acute myocardial injury induced by isoproterenol is modiated by nitric oxide"J.Cardiovase pharmacol. 34. 525-528 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawada T, Shin WS, Nakatsuru Y, et al.: "Precise identification of gene products in hearts after in vivo gene transfection using Sendai virus-coated proteoliposome"Biochem Biophys Res.Commun. 259. 408-413 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawada T, Nakatsuru Y, Sakamoto A, et al.: "Strain-and age-dependent loss of saraylycan complex in cadioryopathic hamster hearts and its re-exprossion by δzalcogycan gene transfer in vivo"FEBS Lett.. 458. 405-408 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki J, Shimamoto R, Nishikawa J, et al.: "Morphological onset and early diagrosis in apital hypertophic cacdio-myopathy: A long term analysis with unclear magnetic resonane ing.ng."J.Am.CoH.Cavdiol. 33. 146-151 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 申 偉秀、豊岡照彦: "Ca拮抗薬のすべて (第二版) 分担分:Ca拮抗薬の血管平滑筋に対する作用"光端医学社. 9/447 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 申 偉秀: "薬物障害ガイド:分担分:血管浮腫"南山堂. 3/ (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wang YP et al: "Nitricoxide(No)Buffers anticellular Ca^<2+>(Ca^<2+>_i)dynamics by its opposite autocine action on inositol 1,4,5-triphosphate(1P_3)induced Celcium release(IICR)& CCE in ECS" Circulation. 98(SupplI). I-667 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 王 岳鵬ら: "血管内皮細胞平滑筋細胞の1,4,5-triphosphate-induced Ca^<2+> releaseの抑制とNonのautocineとparucrine作用" Jpn.Cvc.J.61(SupplI). 283 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki J et al.: "Morphological onset and early diagenesis in apical hyper trophic cardiomyopathy." J.Am Coll Covdol.33. 146-151 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yokoyama I et.al.: "Hypegycemta rather than msulm resstance in related to reducod corouary from ersere in NIDDM." Dabetes. 47. 119-124 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Uehara Y et al.: "A hypertensive father,but not hypertensive mother,determines blood pressure in normotensive male offspring through body mass index" J.Hunan Hypertension. 12. 441-445 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 申 偉秀ら: "ミトコンドリア心筋症を呈した糖尿病の一例" 日本内科学会雑誌. (発表予定). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 申偉秀 豊岡照彦: "総心臓代謝実験法(ICAM-1,VCAM-1の発現の項担当)" 六法出版社, (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 申偉秀: "薬物障害ガッド(血管線腫の項担当)" 南山堂, (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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