| Project/Area Number |
10670686
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
OGASAWARA Yasuo Medical Engineering, Kawasaki Medical School, Associate Professor, 医学部, 助教授 (10152365)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Takeshi Medical Engineering, Kawasaki College of Allied Health Professions, Associate Professor, 臨床工学科, 助教授 (30249560)
YADA Toyotaka Medical Engineering, Kawasaki Medical School, Assistant Professor, 医学部, 講師 (00210279)
KAJIYA Fumihiko Medical Engineering, Kawasaki Medical School, Professor, 医学部, 教授 (70029114)
YAMAMORI Shinji R&D Center, Nihon Kohden Co., Manager, R&Dセンター, 課長
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | NADH / coronary microcirculation / local blood flow / metabolism imaging / micro-strain / molecular tracer / ischaemia |
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| Research Abstract |
We analyzed ischaemia of myocardial micro-circulation and spatial heterogeneity of mitochondrial dysfunction by direct evaluation of NADH fluorescent image in coronary micro-circulation and measurement of capillary blood flow distribution using molecular blood flow tracer. NADH fluorescence imaging system has a CCD camera with 400 thousand pixels to observe the emission fluorescence (420 - 570 nm) against ex-citation ultraviolet ray. The advantages of this system are easy observation by fluorescence for NADH dynamics and high spatial resolution with high time resolution. Blood flow distribution imaging was achieved with molecular blood flow tracer (3H-Desmethylimipramine) with 100 mm spatial resolution. Experiment was performed by 60 seconds hypoxic perfusion in isolated rat heart perfusion preparation. During the experiment, NADH fluorescence from the heart was recorded and molecular blood flow tracers were administered. The findings of the present study were; NADH intensity increased in proportion to the intra-mitochondrial NADH amount immediately after hypoxic perfusion and patchy patterns appeared with mixture of high and low intensity of NADH (several hundreds microns in size). Simultaneous measurement of blood flow distribution by molecular tracers also showed spatial heterogeneity and the size of each spotty area was in order of several hundred microns, which was almost identical with the patch area observed by NADH fluorescence. Furthermore, micro-strain measured at the level of myocardial micro-circulation with the size of a hundred microns also showed heterogeneity on the surface of a heart. Taken together, there is a relationship in distribution among blood flow, metabolic dysfunction and micro-strain. This indicates that hetrogenous myocardial ischaemic area is closely related with blood distribution and mitochondrial metabolic dysfunction.
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