Role of mast cells and antigen-specific IgE antibodies in the pathogenesis of atopic dermatitis
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Single-year Grants|
|Research Institution||Hiroshima University|
MORITA Eishin University medical Hospital, Department of Dermatology, Hiroshima University, Assistant Professor, 医学部・附属病院, 講師 (90182237)
|Project Period (FY)
1998 – 1999
Completed(Fiscal Year 1999)
|Budget Amount *help
¥2,300,000 (Direct Cost : ¥2,300,000)
Fiscal Year 1999 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1998 : ¥1,400,000 (Direct Cost : ¥1,400,000)
|Keywords||atopic dermatitis / IgE / fur mites / NC mouse / mast cells / Candida|
We investigated the role of mast cells and IgE in the pathogenesis of atopic dermatitis.
1. The role of antigen-specific IgE in the development of skin lesions in atopic dermatitis model mouse, NC.
(1) The role of fur mites.
Fur mite-infection resulted in skin lesions in NC associated with an elevated serum IgE, whereas no obvious skin lesions were observed in BALB/C and C57BL/6, and the elevation of serum IgE level was minimal in these two strains of mice. The existence of specific IgE antibody to fur mite-antigen in the serum of mite-infested NC was detected by the antigen-induced histamine release.
(2) The role of serum IgE.
The IgE was purified from the serum of mite-infested NC, and transferred into BALB/C mice. No difference was observed between the NC IgE-transferred BALB/C and control IgE-transferred BALB/C.
2. The role of mast cells.
The mast cell-depleted NC, NC/Kuj-WィイD1vィエD1/WィイD1vィエD1, that lacks the signal transduction of c-kit, were tested to develop dermatitis when infected by
fur mites. Fur mite-infection induced the NC/Kuj-WィイD1vィエD1/WィイD1vィエD1 mice to develop skin lesions indistinguishable to the skin lesions in NC/Kuj-+/+ kept in fur mite-infested conditions, although the acanthosis was relatively mild in NC/Kuj-WィイD1vィエD1/WィイD1vィエD1.
3. The role of immediate hypersensitivity reaction to Candida-antigen
we evaluated skin reaction to Candida-antigen, as well as serum IgE antibody against Candida in the patients with atopic dermatitis, the patients with nasal allergy and non-atopics. The clinical effect of antifungal drugs was estimated in the patients with atopic dermatitis. Immediate hypersensitivity to Candida-antigen is strongly connected with the patients with atopic dermatitis. The delayed type-hypersensitivity to this antigen, which is highly prevalent to atopics without dermatitis as well as non-atopics, was reduced in most of these patients. Antifungal drugs markedly improved the skin manifestation in the patients with atopic dermatitis that have IgE antibodies against Candida in association with a decrease of serum IgE levels. Less
Research Output (13results)