|Budget Amount *help
¥2,800,000 (Direct Cost : ¥2,800,000)
Fiscal Year 1999 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1998 : ¥2,300,000 (Direct Cost : ¥2,300,000)
1) We performed alaSTAT AlaBLOT Specific lg E Assay using sera of 33 cases with latex allergy. We found several bands between 14.7kD and 113kD. The extract of a commercial latex glove inhibited most of these bands.
2) Immunoblot assay against the commercial latex glove using the same sera showed several bands. The extract inhibited one band below 7.2kD, and 28, 37 and 49 kDs bands. It inhibited 23kD band of non-ammoniated latex immunoblot using the same sera.
3) The extract of the commercial latex glove and non-ammoniated latex had common bands, one below 7.2kD, and three bands at 15, 30, and 51kD.
These results showed that our cases had two or three major latex allergens. They are Hev b 6.03 or Hev 8 of 14.7kD, and a band of 30kD which is related to latex fruit syndrome. Two band against the latex glove extract were inhibited by the latex glove extract itself. One was below 7.2kD and the other was 37kD. We suppose that they are Hev b 6.02(hevein, 47.kD) and Hev b 3(βー13-glucanese, 37kD). Inhibition immunoassay against latex glove or related fruits using havein, the related fruits or vegetables will show the mechanism of cross-reactivity between latex and related plants.
2)ラテックス手袋抽出液のimmunoblotを抽出液で抑制した場合,7.2kD以下の分子量のバンドと37kDのバンドが抑制された.これは主要抗原であるHev b6.02(hevein,4.7kD),Hev b3(β-1,3-glucanase,37kD)である可能性が高いと考えた.