|Budget Amount *help
¥3,300,000 (Direct Cost : ¥3,300,000)
Fiscal Year 1999 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1998 : ¥2,700,000 (Direct Cost : ¥2,700,000)
The tumor has large area of hypoxic fraction and low nutrition due to the low vascularity. So tumor tissue produce the vascular endotherial growth factor (VEGF) for angiogenesis. When give the hyperthermia treatment to the tumor, tumor vessels induce the heat damage and increase the hypoxic area. Therefore, the tumor tissue emit the VEGF and then VEGF combined with VEGF-Receptor (VEGF-R) of vessels. When inject the VEGF to near the tumor, the vessels start the angiogenesis to the area of the VEGF injection point. Also, tumor tissue receive the signals of anngiogenesis, and then start the tumor cell proliferation. But still low blood flow and low nutrition, so tumor can't cell proliferation of normal and occur the abnormal division, division delay and cell death. I couldn't cloning the VEGF and VEGF-R. But I extracted the VEGF fraction of the protein from the endotherial cells. When injected the VEGF fraction to near of the tumor, tumor shows the growth delay, and angiogenesis was occurred around injection of the area of VEGF fraction. It means that VEGF can enhance the hyperthermia effect, and useful for treatment of the brain infaction and other infaction disease.