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Analysis of Islet Development Using Transgenic Mice

Research Project

Project/Area Number 10671036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionThe University of Tokushima

Principal Investigator

YAMAOKA Takashi  The University of Tokushima, Institute for Genome Research, Division of Genetic Information, Associate Professor, ゲノム機能研究センター, 助教授 (40263826)

Co-Investigator(Kenkyū-buntansha) SUGINO Hiromu  The University of Tokushima, Institute for Enzyme Research, Professor, 分子酵素学研究センター, 教授 (50211305)
NOJI Sumihare  The University of Tokushima, Department of Biological Science and Technology, Professor, 工学部, 教授 (40156211)
ITAKURA Mitsuo  The University of Tokushima, Institute for Genome Research, Division of Genetic Information, Professor, ゲノム機能研究センター, 教授 (60134227)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsPancreatic islets / Pancreatic β cells / Transgenic mouse / TGF-β / Activin / Interferon-γ / Pax6 / Reg I / 糖尿病 / アポトーシス / 腫瘍発生 / 発生工学 / サイトカイン / Pax-6
Research Abstract

(1) Glucagon promoter/active TGF-β-transgenic NOD mouse : The rate of onset of type 1 diabetes decreased because of T-cell suppression by TGF-β, and islet hypoplasia was induced by the suppressive effect of TGF-β on the growth of islets.
(2) Insulin promoter/dominant negative, constitutively active, or normal activin receptor-transgenic mice : In transgenic mice expressing activin-receptor mutants, islets were hypoplastic, whereas transgenic mice expressing normal activin receptor did not show any abnormality. Therefore, appropriate intensity of activin signal is necessary for normal development of islets.
(3) Glucagon promoter/interferon-γ-transgenic mouse : Interferon-γ directly impaired β cells without lymphocytes and stimulated the proliferation of immature β-cells, leading to islet remodeling.
(4) Insulin promoter or Pdx1 promoter/Pax6-transgenic mice : In insulin promoter/Pax6-transgenic mice, β-cell differentiation was impaired. In Pdx1 promoter/Pax6-transgenic mice, epithelial hyperplasia of pancreatic ducts and cystic adenoma were observed, in addition to the decrease in the number of β cells. It was suggested that Pax6 has a dual action on islet development : one is the stimulation of proliferation of islet precursor cells and the other is the induction of α-cell phenotypes.
(5) Glucagon promoter/Reg I-transgenic mouse : Overexpression of Reg I induced β-cell apoptosis, and diabetes. Furthermore, excessive secretion of Reg I resulted in diverse malignant tumors. The tumor-promoting activity of Reg I protein should be considered for its possible clinical applications.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takashi Yamaoka: "Hypoplasia of Pancreatic Islets in Transgenic Mice Expressing Activin Receptor Mutants"J.Clin.Invest.. 102・2. 294-301 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maki Moritani: "Abrogation of Autoimmune Diabetes in Nonobese Diabetic Mice and Protection against Effector Lymphocytes by Transgenic Paracrine TGF-β1"J.Clin.Invest.. 102・3. 499-506 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka: "Development of Pancreatic Islets (Review)."Int.J.Mol.Med.. 3. 247-261 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka: "Apoptosis and Remodeling of Beta-cells by Paracrine Interferon-γ without Insulitis in Transgenic Mice"Diabetologia. 42. 566-573 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka: "Diabetes and Pancreatic Tumours in Transgenic Mice Expressing Pax 6"Diabetologia. 43・3. 332-339 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka: "Diabetes and Tumor Formation in Transgenic Mice Expressing Reg I"Biochem.Biophys.Res.Commun.. 278. 368-376 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka, et al.: "Hypoplasia of Pancreatic Islets in Transgenic Mice Expressing Activin Receptor Mutants."J.Clin.Invest.. 102-2. 294-301 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maki Moritani, et al.: "Abrogation of Autoimmune Diabetes in Nonobese Diabetic Mice and Protection against Effector Lymphocytes by Transgenic Paracrine TGF-β1."J.Clin.Invest.. 102-3. 499-506 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka, et al.: "Development of Pancreatic Islets (Review)."Int.J.Mol.Med.. 3. 247-261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka, et al.: "Apoptosis and Remodeling of Beta-cells by Paracrine Interferon-γ without Insulitis in Transgenic Mice."Diabetologia. 42. 566-573

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka, et al.: "Diabetes and Pancreatic Tumours in Transgenic Mice Expressing Pax6."Diabetologia. 43-3. 332-339 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka, et al.: "Diabetes and Tumor Formation in Transgenic Mice Expressing Reg I."Biochem.Biophys.Res.Commun.. 278. 368-376 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Yamaoka: "Diabetes and Pancreatic Tumours in Transgenic Mice Expressing Pax 6"Diabetologia. 43・3. 332-339 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takashi Yamaoka: "Diabetes and Tumor Formation in Transgenic Mice Expressing Reg I"Biochem.Biophys.Res.Commun.. 278. 368-376 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kenji Sasahara: "Molecular Cloning of a New Member of the Reg Family, INGAP-related Protein"Biochim.Biophys.Acta. 1500. 142-146 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kenji Sasahara: "Molecular Cloning and Expression Analysis of a Putative Nuclear Protein SR-25"Biochem.Biophys.Res.Commun.. 269. 444-450 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Maki Kondo : "The Rate of Cell Growth Is Regulated by Purine Biosynthesis via ATP Production and G1 and S Phase Transition"J.Biochem.. 128. 57-64 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tohru Kikutsuji: "Dominant Expression of Type-2 Somatostatin Receptor Subtype in Human Exocrine Pancreatic Cancers"J.Hepatobiliary Pancreat.Surg. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Takashi Yamaoka: "Diabetes and pancreatic Tumours in Transgenic Mice Expressing Pax6."Diabetologia. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takashi Yamaoka: "Apoptosis and Remodeling of β-cells by paracrine IFN-γ without insulitis in transgenic mice."Diabetologia. 42. 566-573 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takashi Yamaoka: "Hypoplasia of pancreatic islets in transgenic mice expressing activin receptor mutants."J.Clin.Invest.. 102・2. 294-301 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Maki Moritani: "Abrogation of autocrine diabetes in nonobese diabetic mice and protection against effector lymphocytes by TGF-β1."J.Clin.Invest. 102・3. 499-506 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takaya Matsushita: "Molecular cloning of mouse Pax-4 from anislet βcell line and deduced sequence of human Pax-4."Biochem.Biophys.Res.Commun.. 242. 176-180 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chisato Tanaka: "Analysis of LOH in chromosome 11 and infrequent inactivation of the MEN1 gene in sporadic pituitary adenoma."J.Clin.Endocrinol.Metab.. 83・8. 2631-2634 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takashi Yamaoka: "Development of Pancreatic Islets"Lychnia Athens. 15 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takashi Yamaoka: "Electrophoresis of Proteins and Peptides"Elsevier. 306 (1998)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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