THE STUDY FOR THE PHISIOLOGICAL ROLES OF HDL BINDING PROTEIN(HBィイD22ィエD2)ON THE PROGRESSION OF ATHEROSCRELOSIS
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Single-year Grants|
|Research Institution||JIKEI UNIVERSITY SCHOOL OF MEDICINE|
KURATA Hideaki JIKEI UNIVERSITY SCHOOL OF MEDICINE, 医学部, 助手 (00287228)
|Project Period (FY)
1998 – 1999
Completed(Fiscal Year 1999)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1999 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1998 : ¥1,300,000 (Direct Cost : ¥1,300,000)
|Keywords||LIPOPROTEIN RECEPTOR / HDL / HYPERLIPIDEMIA / ATHEROSCLEROSIS / HDL-RECEPTOR / MACROPHAGE / ADHESION MOLECULE / アセチルLDL / 糖尿病 / 高比重リポ蛋白(HDL)|
HDL binding protein (HBィイD22ィエD2), a glycoprotein of Mr 100 kDa was purified from rat liver plasma membrane. This protein shows high homology with adhesion molecules ALCAM and BEN.
1) Tissue distribution of HBィイD22ィエD2 in rat.
We obtained rat HBィイD22ィエD2 DNA fragment from cDNA library of rat lung with PCR method, and studied tissue distributions of HBィイD22ィエD2 in rat with Northern blot analysis. On Northern blot analysis, HィイD22ィエD2 mRNA expression in various rat tissues. Strongest expression was found in the lung, then brain, liver and kidney.
2) Messenger RNA expressions of HBィイD22ィエD2 in THP-1 cell and macrophage.
To determine whether blood monocytes or macrophages express HBィイD22ィエD2, RNA from THP-1 cells differentiated by treatment with PMA was subjected to Northern blot analysis. HBィイD22ィエD2 mRNA, very low in untreated THP-1 cells, was strongly induced when the cells were transformed into macrophages by PMA treatment.
To investigated whether HBィイD22ィエD2 was sensitive to the cellular cholesterol content we incubated transformed THP-1 cells with 50 or 100 μ g/ml of acetylated LDL in medium. A dose dependent reduction in HBィイD22ィエD2 mRNA expression was observed in the acetylated LDL treated cells.
3) Messenger RNA expression of HBィイD22ィエD2 in rat mesangial cell.
Rat mesangial cells were prepared with seaving methods from male Wistar rat. Rat mesangial cells were cultured with RPMI 1640 medium and were also treated with PMA for 12 hours. HBィイD22ィエD2 Mrna, very low in untreated mesangial cells, was not induced when the cells were treated with PMA.
Research Products (7results)