Experimental studies on elimination of hyperinsulinemia after pancreas transplantation
Project/Area Number |
10671127
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
NAKAI Ichiro Kyoto Prefectural University of Medicine, Transplant and Endocrine Surgery, Assistant Professor, 医学部, 助手 (30188867)
|
Project Period (FY) |
1998 – 1999
|
Project Status |
Completed (Fiscal Year 1999)
|
Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
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Keywords | Pancreas / Transplantation / Hyperinsulinemia / Portal drainage / Insulin receptor / Rat / Pancreas Transplantation / 膵移植 / 高インスリン血症 / インスリン抵抗性 / 門脈ドレナージ |
Research Abstract |
Whole pancreaticoduodenal transplantations with portal (PD) and systemic venous drainage were achieved in the streptozotocin-induced diabetic rats. Porto-peripheral gradient of insulin levels during IV-GTT was abrogated in the SD group, though it was well sustained in the PD group. The SD group revealed high ketone bodies, high LDL, and low HDL levels. As pancreas transplant with PD can normalize metabolic condition generally, the portal vein is preferable site for insulin delivery. However, PD could not resolve hyperinsulinemia completely. In the next step, we examined the effect of troglitazone (TGZ) on insulin regulation after pancreas transplantation. The rations of ΣIRI/ΣPG (plasma glucose) during IV-GTT were calculated as a parameter for insulin resistance, and the value in TGZ-treated rats (0.23【plus-minus】0.11μ U/ml : mg/dl) was lower than in TGZ-free rats (0.35【plus-minus】0.05μ U/ml : mg/dl) ; thus TGZ regulated insulin levels in peripheral blood after glucose load in rats bearing pancreas graft with SD. On quantitative analysis of insulin receptor content in the hindlimb muscle, TGZ activated tyrosine-phosphorylation depending on the number of insulin receptor. This fact indicated that TGZ enhanced cellular response to insulin and reduce hyperinsulinemia after pancreas transplantation.
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Report
(3 results)
Research Products
(14 results)