Project/Area Number |
10671166
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Digestive surgery
|
Research Institution | Toyama Medical and Pharmaceutical University |
Principal Investigator |
SAITO Mitsukazu Hospital, Toyama Medical and Pharmaceutical University Assistant Professor, 附属病院, 助手 (30270945)
|
Project Period (FY) |
1998 – 1999
|
Project Status |
Completed (Fiscal Year 1999)
|
Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
Keywords | gastrointestinal cancer cells / hemagglutinin / liposome / gene therapy / anti-oncogene |
Research Abstract |
1) I have attempted to study the cell growth by adenovirus-mediated transfer of the wild-type p53 in human esophageal carcinoma cells using a pannel of 15 sample cells consisting of 8 cell lines and 7 nude mouse-transplantable esophageal caricinomas which were established for culture in vitro and trasplant in nude mice in our laboratory, respectively. Moreover I have attempted to study the cell growth by liposome attached hemagglutinin and included anti-oncogene in human esophageal carcinoma cells using a pannel of 15 sample cells. 2) As a result, minor difference occurred in the results during the these cell lines, but all cell lines showed suppressive tendency. This tendency was recognized in case of using by adenovirus, and more suppressive tendency was recognized in case of using by liposome. However, I am not able to obtaine stable liposome. 3) To further studies, esophageal carcinoma tissues haved been collected from several patients and established as culturable cell lines and transplantable carcinomas in nude mice. Sera of the patients have also been collected and stored.
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