Project/Area Number |
10671192
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Digestive surgery
|
Research Institution | The University of Tokushima |
Principal Investigator |
NISHI Masaharu The University of Tokushima, Hospital, Associate Professor, 医学部・附属病院, 講師 (40228143)
|
Project Period (FY) |
1998 – 2000
|
Project Status |
Completed (Fiscal Year 2001)
|
Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
Keywords | indigenous bacteria / bacterial translocation / obstructive jaundice / liver resection / clearance mechanism / intestinal structure / bile acid / bile / 遺伝標識付細菌 / バクテリアルトランスローケイション / クリアランス機能 / 遺伝表識付細菌 / 肝切除術 |
Research Abstract |
In this research project, we developed the indigenous bacteria and named JNW14. JNW14 originally isolated from Wistar rat feces was labeled with bacitracin-, neomycin-and streptomycin-resistant markers. The method using indigenous bacteria (JNW14) was useful to study for bacterial translocation (BT). The animal study using JNW14 identified the BT system in obstructive jaundice. Major liver resection under obstructive jaundice promoted BT to systemic organs and the number of viable JNW14 in the mesenteric lymph nodes in the lung culture positive group was significantly higher than that of negative group, suggesting the major route of BT is lymphatic-thoracic system. The reticuloendothelial system function was impaired in obstructive jaundice and hepatectomy, and it was mainly due to the decrease of the uptake of bacteria in the liver. Morphology of the ileum showed structural abnormalities and increased intestinal permiability in obstructive jaundiced rat. The administration of cholic acid and deoxycholic acid were not decreased BT, but the whole bile decresed intestinal permiability and inhibited BT under obstrustive jaundice state.
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