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A study for mechanism of lymphatic metastasis with human gastric cancer cell line

Research Project

Project/Area Number 10671202
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionSAPPORO MEDICAL UNIVERSITY

Principal Investigator

DENNO Ryuichi  SAPPORO MEDICAL UNIVERSITY SCHOOL OF HEALTH SCIENCES, PROFESSOR, 保健医療学部, 教授 (40163943)

Co-Investigator(Kenkyū-buntansha) URA Hideki  SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE ASCISTANT PROFESSOR, 医学部, 講師 (50264510)
SATO Takashi  SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE, ASOCIATE PROFESSOR, 医学部, 助教授 (50205928)
HIRATA Koichi  SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (50136959)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
KeywordsAZ521 / peritoneal metastasis / lymphatic metastasis / integrins / CD44 / human gastric cell line / ヒト胃癌細胞株AZ521 / ヒト膵癌細胞株HPC-3 / 血管新生抑制剤 / TNP-470 / 腹膜播腫 / 肝転移 / 高肝転移株AZ-H5G / 高リンパ節転移株AZ-L5G
Research Abstract

Though lymph node metastasis is very often encountered in patients with gastric cancer, the biological mechanism of lymph node metastasis is not clarified well, compared with hematogenous and peritoneal metastasis. The animal model of lymph node metastasis is rarely established and reported. We have already established highly liver metastatic mice model, AZ-H7d and peritoneal metastatic model, AZ-P7a, from the parental AZ-521 lines with a low potential of metastasis and investigated the mechanisms of these two types of metastasis. Our results suggested that enhanced expression of VEGF and α3-integrin in hematogenous metastasis, enhanced motile activity and α5-, α v β 5-integrins in peritoneal dissemination might play an important role in gastric cancer. At the basis of these experiments, we established novel mice model with high potential of lymph node metastasis, designated as AZ-L5G.In comparison with AZ-521, we investigated the biology implicated the development of lymph node metastasis. Then the entity that makes the biological differences of lymph node metastasis and hematogenous and/or peritoneal metastasis was investigated compared with AZ-H7d and AZ-P7a. Moreover, recent development of molecular biology revealed that a variety of genes were concerned with the cancer metastasis at their each steps. Using cDNA macroarray and DNA chip technologies, the relative mRNA levels of genes expressed in AZ-521 and AZ-L5G cell lines are identified. Moreover, we perform global analysis on different gene expression of AZ-521, AZ-H7d, AZ-P7a and AZ-L5G.This unique, in vivo model cell line might be highly useful to study the biology of metastasis of gastric cancer and our results lead to the development of new therapeutic modalities against human gastric cancer.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Ryuichi Denno, et al.: "The relationship between the expressions of the cell adhesion molecule CD44,CD44v3, and CD44v6 and metastases in gastric cancer."Tumor Res. 33. 35-44 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayuki Shishido, et al.: "Inhibition of liver metastasis of human pancreatic carcinoma by angiogenesis inhibitor TNP-470 in combination with cisplatin."Jpn J Cancer Res. 89. 963-969 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shishido T., et al.: "Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice."Surg Today. 29. 519-529 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimori H., et al.: "A Novel Experimental Mouse Model of Peritoneal Dissemination of Human Gastric Cancer Cells. Different Mechanisms in Peritoneal Dissemination and Hematogenous Metastasis."Jpn J Cancer Res. 91. 715-722 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamaguchi K., et al.: "Establishment and Characterization of a Human Gastric Carcinoma Cell Line that is Highly Metastatic to Lymph Nodes."J Exp Clin Cancer Res. 19. 113-120 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ryuichi Denno, Hideki Ura, Koichi Hirata, Takahiro Yasoshima, Takayuki Shishido, Koji Yamaguchi.: "The relationship between the expressions of the cell adhesion melecule CD44, CD44v3, and CD44v6 and metastases in gastric cancer."Tumor Res. 33. 35-44 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayuki Shishido, Takahiro Yasoshima, Ryuichi Denno, Mitsuhiro Mukaiya, Noriyuki Sato, Koichi Hirata.: "Inhibition of liver metastasis of human pancreatic carcinoma by angiogenesis inhibitor TNP-470 in combination with cisplatin."Jpn J Cancer Res. 89. 963-969 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayuki Shishido, Takahiro Yasoshima, Koichi Hirata, Ryuichi Denno, Mitsuhiro Mukaiya, Hideki Ura, Koji Yamaguchi, Satoru Kawaguchi, Noriyuki Sato.: "Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice."Surgery Today. 29. 519-529 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hidefumi Nishimori, Takahiro Yasoshima, Ryuichi Denno, Takayuki Shishido, Fumitake Hata, Yojiro Okada, Hideki Ura, Koji Yamaguchi, Hiroshi Isomura, Noriyuki Sato, and Koichi Hirata.: "A Novel Experimental Mouse Model of Peritoneal Dissemination of Human Gastric Cancer Cells. Different Mechanisms in Peritoneal Dissemination and Hematogenous Metastasis."Jpn J Cancer Res. 91. 715-722 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koji Yamaguchi, Hideki Ura, Takahiro Yasoshima, Takayuki Shishido, Ryuichi Denno, and Koichi Hirata.: "Establishment and Characterization of a Human Gastric Carcinoma cell Line that is Highly Metastatic to Lymph Nodes."J Exp Clin Cancer Res. 19. 113-120 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shishido T.,Yasoshima T., et al.: "Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice."Surg Today. 29. 519-529 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishimori H.,Yasoshima T., et al.: "A Novel Experimental Mouse Model of Peritoneal Dissemination of Human Gastric Cancer Cells.Different Mechanisms in Peritoneal Dissemination and Hematogenous Metastasis."Jpn J Cancer Res. 91. 715-722 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamaguchi K.,Ura H., et al.: "Establishment and Characterization of a Human Gastric Carcinoma Cell Line that is Highly Metastatic to Lymph Nodes."J Exp Clin Cancer Res. 19. 113-120 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shishido T.,et al.: "Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice."Surg Today. 29. 519-529 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takayuki Shishido,et al.: "Inhibitation of Liver Metastasis of Human Pancreatic Carcinoma by Angiogenesis Inhibitor TNP-470 in Combination with Cisplatin" Jpn.J.Cancer Res.89. 963-969 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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