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Research about proteasome inhibitors-induced apoptosis in human glioma cells

Research Project

Project/Area Number 10671333
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionHyogo College of Medicine

Principal Investigator

NAKANO Atsuhisa  Hyogo College of Medicine, Faculty of Medicine, Assistant Professor, 医学部, 助手 (70258151)

Co-Investigator(Kenkyū-buntansha) 中野 敦久  兵庫医科大学, 医学部, 助手 (70258151)
KITAGAWA Hiroyuki  
池本 秀康  兵庫医科大学, 医学部, 助手 (30278824)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsProteasome / Apoptosis / Glioma / ミトコンドリア / プロテアソーム阻害剤 / ヒト・グリオーマ / カスペースファミリー / Bcl-2ファミリー
Research Abstract

Proteasome inhibitors lactacystin and AcLLNal induced p53-independent apoptosis in two human glioma cell lines, and the apoptosis was accompanied by up-regulation of immunoreactive wild-type p53, p21ィイD1Waf1ィエD1, Mdm2, and p27ィイD1Kip1ィエD1. The pretreatment with cycloheximide decreased the induction of cell death independently of p53 protein status, suggesting that the up-regulation of short-lived proteins is associated with proteasome inhibitor-induced apoptosis. Caspase-3-like proteases were activated in the proteasome inhibitor-mediated apoptosis, and the induction of cell death was inhibited more effectively in the presence of z-VAD.fmk than in the presence of Ac-DEVD.fmk, suggesting that caspases other than caspase-3 are involved. Nonetheless, there was no significant alterations in levels of immunoreactive Bcl-2, Bcl-xィイD2LィエD2, Bax, Bad, and Bak, as well as no evidence of cyt c release into cytosol and dissipation of ΔψィイD2mィエD2. Thus, the proteasome inhibitor-induced apoptosis is mediated by mitochondria-independent mechanism, and the once activated caspase-3 does not cause the cyt c release and the ΔψィイD2mィエD2 disruption.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Hiroyuki Kitagawa: "Proteasome inhibitors inducc mitochondria-independent apoptosis in human glioma cells"FEBS Letters. 443. 181-186 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitagawa H, Tani E, Ikemoto H, Ozaki I, Nakano A, Omura S.: "Proteasome inhibitors induce mitochondria-independent apoptosis in human glioma cells"FEBS Lett. 443. 181-186 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroyuki Kitagawa: "Proteasome inhibitors lnduce mitochondria-independent apoptosis in human glioma cells"FEBS Letters. 443. 181-186 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitagawa H: "Proteasome inhibitors induce mitochondria-independent apoptosis in human glioma cells" FEBS Letters. 443. 181-186 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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