Purposes : The influences of propofol over liver and renal function have not been studied in vitro. Using precision-cut tissue slices in dynamic organ culture system, we investigated whether propofol displays hepatotoxicity or nephrotoxicity during hypoxia-reoxygenation.
Methods : Male Wistar ras were used. Liver and kidney slices (200μm thick), precision-cut on a Brendel/Vitron tissue slicer, were preincubated for 2 hr in Waymouth's mediumn. In the oxygenation groups tissue slices were iucubated in Waymoumth's medium containing vatious concentrations of propofol and gassed with 95%O_2/5%CO_2. In the hypoxia -reoxygenation groups, slices were first incubated in Waymouth's medium gassed with l00%N_2, then in medium reoxygenated with 95%O_2/5%CO_2 gas, both incubation media containing various concentrations of propofol. Slice viability was assessed over a 4-hr period by measuring intracellular K^+ content, ALT, AST, and alpha GST lcakagc liver slices or intracellular K^+ content, NAG, gamma GTP, and alpha GST leakage kidney slices.
Results : In the oxygenation groups, the intracellular K^+ content of liver and kidney slices was maintained throughout the incubation period with or without propofol, and there were no differences in enzyme leakage among the experimental groups during the incubation. In contrast, in the hypoxia -reoxgenation groups the tissue K^+ content was lower in the propofol groups than in the no propofo group. Enzyme leakage was greatr in the propofol groups than in the no propofol group during hypoxia-reoxygenation.
Summamy : In precision-cut tissue slices in a dynamic organ-culture system, propofol does not exhibit hepatotoxicity or nephrotoxicity under oxygenated conditions. However, our results suggest that propofol may have effects on membrane integrity in the liver and kidney during hypoxia-reoxygenation.