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MORPHOMETRIC AND IMMUNO-ELECTRON MICROSCOPIC STUDY OF NON-FUNCTIONING ADRENOCORTICAL ADENOMAS IN COMPARISON WITH FUNCTIONING ADRENOCORTICAL ADENOMAS

Research Project

Project/Area Number 10671499
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNihon University

Principal Investigator

HIRANO Daisaku  NIHON UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (40228804)

Co-Investigator(Kenkyū-buntansha) ISHIDA Hajime  NIHON UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (70138452)
OKADA Kiyoki  NIHON UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (70059301)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
KeywordsNon-functioning adrenocortical adenoma / Aldosterone-producing adenoma / adenoma with Cushing's syndrome / Ultrastructure / Morphometry / Immuno-electron microscopic study / プレクリニカルクッシング腺腫
Research Abstract

We performed electron microscopic studies of 11 non-functioning adrenocortical adenomas (NFA), 2 adenomas with pre-clinical Cushing's syndrome, 10 aldosterone-producing adenomas (APA) and 4 adenomas with Cushing's syndrome, obtained by surgical extirpation. Ultrastructurally, 9 of 11 NFA were classified into the aldosteron-producing type and 2 into the Cushing's type. The ultrastructural features of adenomas with pre-clinical Cushing's syndrome were similar those of adenomas with Cushing's syndrome.
Based on morphometric analysis, the mean ratio and standard deviation (SD) of mitochondria (M), smooth-surface endoplasmic reticulum (SER), rough-surface endoplasmic reticulum (RER), lipid vacules (LV) and lysosomes (Ly) was 11.2 ± 7.7%, 16.3 ± 13.8%, 2.8 ± 2.0%, 20.0 ± 23.5% and 1.3 ± 1.9% in the NFA, and 17.2 ± 8.6%, 19.3 ± 9.8%, 6.3 ± 5.5%, 19.4 ± 13.1% and 1.2 ± 1.6% in the APA, respectively. The ratio of M (p<0.01), SER (p<0.01) and RER (p<0.01) in the NFA significantly declined compared to that in the APA.
The ratio of LV in NFA resembling adenomas with Cushing's syndrome and adenomas with pre-clinical Cushing's syndrome significantly increased compared to that of adenomas with Cushing's syndrome. The ratio of M and SER in the NFA resembling adenomas with Cushing's syndrome significantly decreased compared to that in adenomas with Cushing's syndrome, whereas the ratios of these organelles between adenomas with Pre-Cushing syndrome and those with Cushing's syndrome were not statistically different.
The difference of p-450scc localization among NFA, APA and adenomas with Cushing's syndrome was not found based on immuno-electron microscopic study.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 平野大作他: "内分泌非機能性副腎皮質腺腫とアルドステロン腺腫の電顕的形態計測法と組織内ホルモン定量による比較検討"日本泌尿器科学会雑誌. 89.2. 237-237 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 平野大作他: "Preclinical Cushing症候群を呈した副腎皮質腺腫の電顕的観察(Cushing腺腫との比較検討)"日本臨床電子顕微鏡学会誌. 32、増刊号. 107-107 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano D et al.: "Morphometric analysis, at electron microscope level, combined with hormone assay of nonfunctioning adrenocortical adenoma : comparison with aldosterone-producing adenomas"Medical Electron Microscopy. 34. 234-243 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano D et al.: "Electron microscopic study to compare preclinical Cushing's Syndrome with overt Cushing's syndrome"International Journal of Urology in press 2002.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano D. et al.: "Morphometric and quantitative Analysis of Non-functioning adenomas : comparison with Aldosterone producing adenomas"Japanese Journal Urology. 89(2). 237 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano D et al.: "Electron microscopic study of Adenomas with Pre-clinical Cushing syndrome in comparison with adenomas with Cushing syndrome"Japanese Clinical Electron Microscopy. 32 supplement. 107 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano D. et al.: "Morphometric analysis,at electron microscope level, combined with hormone assay of nonfunctioning adrenocortical adenoma : comparision with aldosterone-producing adenomas"Med Electron Microsc. 34(4). 234-243 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano D et al.: "Electron microscopic study, to compare preclinical Cushing's syndrome with overt Cushing's syndrome"International Journal of Urology. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 平野大作他: "Preclinical Cushing症候群を呈した副腎皮質腺腫の電顕的観察(Cushing腺腫との比較検討)"日本臨床電子顕微鏡学会誌. 32、増刊号. 107-107 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 平野大作 他: "内分泌非機能性副腎皮質腺腫とアルドステロン腺腫の電顕的形態計測法と組織内ホルモン定量による比較検討" 日本泌尿器科学会雑誌. 89・2. 237-237 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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