• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of elastase and matrix metalloproteinases in pseudomonal keratitis

Research Project

Project/Area Number 10671647
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKumamoto University School of Medicine

Principal Investigator

MATSUMOTO Koki  Kumamoto University Ophthalmology Associate Professor, 医学部, 助教授 (70173896)

Co-Investigator(Kenkyū-buntansha) HIRATA Akira  Kumamoto University Ophthalmology, 医学部・附属病院, 助手 (60295144)
MURATA Yasuhiro  Kumamoto University Ophthalmology, 医学部, 助手 (80229997)
MIYAGAWA Shin-ichi  Kumamoto University Ophthalmology, 医学部, 助手 (10260738)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPseudomonas aeruginosa / Corneal ulcer / elastase / Keratocyte / MMP-2 / MMP-9 / protease / neutrophil / 緑膿菌性角膜炎におけるエラスターゼ及びMMPの動態と役割 / アルカリプロチアーゼ
Research Abstract

Major pathogenic factors that contribute to ulceration in pseudomonal keratitis could be proteases. We investigated the role of pseudomonal elastase and MMPs in pseudomonal keratitis. Pseudomonal keratitis was produced in animals by injecting live bacteria into the corneas and proteases produced in the corneas were analyzed with gelatin and casein zymography. Several MMPs and caseinases with molecular weight of 24-27 kDa were detected. The MMPs included MMP-2 (gelatinase A), MMP-9 (gelatinase B), activated forms of these MMPs and gelatinase with molecular weight of >200 kDa. However, pseudomonal elastase was not detected. In order to clarify the origin of these proteases, culture media conditioned by corneal stromal cells with or without pseudomonal elastase were analyzed with zymography. MMP-2 was detected if the corneal cells were cultured without pseudomonal elastase. However, MMP-2, MMP-9 and activated forms of these MMPs were detected when pseudomonal elastase was added to the medium. Neutrophil extracts contained MMP-9, gelatinase with molecular weight of >200 kDa and caseinase with molecular weight of 24-27 kDa. Immunohistochemistry (ABC method) showed the presence of pseudomonal elastase in the corneas. From these results it is indicated that proteases that contribute to ulceration could be derived from bacteria, corneal cells and neutrophil, and the MMPs can be activated by pseudomonal elastase.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Koki Matsumoto: "Lecthin-bound superoxide dismutase in the prevention of neutrophil-induced damage of corneal tissue"Invest. Opthalnol. Vis. Sci.. 39(1). 30-35 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本光希: "緑膿菌と角膜感染症"あたらしい眼科. 15. 95-100 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigeo Shimmura: "Acidic electrolysed water in te disinfection of the oculer surface"Exp. Eye Res.. 70. 1-6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koki Matsumoto: "proteases in bacterial keratitis"Cornea (supplement). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto K, Shimmura S, Goto E, Saito K, Takeuchi T, Miyajima S, Negi A, Tsubota K: "Lecithin-bound superoxide dismutase in the prevention of neutrophil-induced damage of corneeal tissue"Invest Ophthalmol Vis Sci. 39. 30-35 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimmura S, Matsumoto K, Yaguchi H, Okuda T, Miyajima S, Negi A, Shimazaki J, Tsubota K: "Acidic electrolysed water in the disinfection of the ocular surface"Exp. Eye Res.. 70. 1-6 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto K: "Proteases in bacterial keratitis"Cornea(supplement). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koki Matsumoto: "Lecithin-bound superoxide dismutase in the prevention of neutrophil-induced damage of corneal tissue"Invest.Ophthalmol.Vis.Sci. 39(1). 30-35 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shigeto Shimmura: "Acidic electrolysed water in the disiafection of the ocular surface"Exp.Eye.Res.. 70(1). 1-6 (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi