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Fundamental research of the effect of a variety of chemotherapeutics on leukocyte functions

Research Project

Project/Area Number 10671759
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka Dental University

Principal Investigator

OHURA Kiyoshi  Osaka Dental University, Dentistry, Professor, 歯学部, 教授 (20131378)

Co-Investigator(Kenkyū-buntansha) AZUMA Yasutaka  Osaka Dental University, Dentistry, Assistant, 歯学部, 助手 (50298816)
SHINOHARA Mitsuko  Osaka Dental University, Dentistry, Associate Professor, 歯学部, 助教授 (40067187)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsnew quinolones / macrophages / neutrophils / superoxide / phagocytosis / chemotaxis / adhesion / hydrogen peroxide / 貧食能 / ラット
Research Abstract

Macrophages and neutrophils play an important role in natural host defense system. When these phagocytes recognize foreign particles, such as bacterium and fungi, they escape the vascular system, migrate towards the site of infection, followed by phagocytosis and intracellular killing of the invading microorganisms by bactericidal reactive oxygen species derived from the superoxide anion. To determine whether antimicrobials affect these functions, we examined the effect of 6 new quinolones, ofloxacin (OFLX), lomefloxacin (LFLX), tosufloxacin (FLRX), fleroxacin (FLRX), sparfloxacin (SPFX) and levofloxacin (LVFX) on the function of rat peripheral macrophages and neutrophils. In vitro treatment of the new quinolones was effective in markedly potentiating the production of superoxide anion in macrophages. The potentiation of the production of superoxide anion is prolonged with TFLX but transient for OFLX, LFLX, FLRX, SPFX and LVFX. In contrast, TFLX markedly potentiated adhesion in macroph … More ages. No significant alteration was detected in the adhesion in macrophages treated with the other new quinolones. In addition, all of the new quinolones significantly inhibited chemotaxis and phagocytosis of E. coli in macrophages. Moreover, OFLX, LFLX, TFLX, and LVFX were effective in significantly potentiating the production of hydrogen peroxide in macrophages, while the other agents did not affect that. By contrast, OFLX, LFLX, FLRX and LVFX caused an increase of phagocytosis of E. coli in neutrophils. However, TFLX and SPFX failed to significantly affect phagocytosis in neutrophils. Furthermore, LFLX and SPFX significantly potentiated adhesion of neutrophils. A potentiation by TFLX was seen with the production of superoxide anion in neutorphils, while the other five new quinolones were effective in markedly reducing that production in neutrophils. Moreover, TFLX was effective in significantly potentiating the production of hydrogen peroxide in neutrophils, whereas sparfloxacin markedly inhibited such production. Therefore, the new quinolones may differentially affect the function of macrophages and neutrophils. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 東 泰孝: "ニューキノロン薬のマクロファージ機能に及ぼす影響"日本薬理学雑誌. 113(3). 48P (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Interaction between new quinolones and functions of macrophages"Journal of Dental Research. 78(Special Issue). 183 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Possible interaction between new quinolones and immune functions in macrophages"General Pharmacology. 32(5). 609-614 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Comparative studies of modulatory effect to the function of rat peritoneal neutrophils treated with new quinolones"Immunology Letters. 69(3). 321-327 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Effect of new quinolones on neutrophils"Japanese Journal of Pharmacology. 78(SI). 49P (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Possible interaction of new quinolones with phagocytes"Current Topics in Pharmacology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azuma Y, Shinohara M and Ohura K: "Effect of new quinolones on macrophage functions"Folia pharmacologica japonica. 113(3). 48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azuma Y, Shinohara M and Ohura K: "Interaction between new quinolones and functions of macrophages."Journal of Dental Research. 78(Special Issue). 183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azuma Y, Shinohara M, Murakawa N, Endo M and Ohura K: "Possible interaction between new quinolone and immune functions in macrophages."General Pharmacology. 32(5). 609-614 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azuma Y, Wang P-L, Shinohara M, Okamura M, Inui Y, Suese Y and Ohura K: "Comparative studies of modulatory effect to the function of rat peritoneal neutrophils treated with new quinolones."Immunology Letters. 69(3). 321-327 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azuma Y, Wang P-L, Shinohara M and Ohura K: "Effect of new quinolones on neutrophils."Japanese Journal of Pharmacology. 78(SI). 49 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azuma Y, Shinohara M and Ohura K: "Possible interaction of new quinolones with phagocytes."Current Topics in Pharmacology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Possible interaction between new quinolones and immune functions in macrophages"General Pharmacology. 32(5). 609-614 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasutaka Azuma: "Comparative studies of modulatory effect to the function of rat peritoneal neutrophils treated with new quinolones"Immunology Letters. 69(3). 321-327 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasutaka Azuma: "Effect of new quinolones on neutrophils"Japanese Journal of Pharmacology. 78(SI). 49P (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 東 泰孝: "ニューキノロン薬のマクロファージ機能に及ぼす影響" 日本薬理学雑誌. 113(3). 48P (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasutaka Azuma: "Interaction between new quinolones and fuctions of macrophages" Journal of Dental Research. 78(Special Issue). 183 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasutaka Azuma: "Possible interaction between new quinolones and immune functions of macrophages" General Pharmacology. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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