| Project/Area Number |
10671811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YAMAGUCHI Taihiko Hokkaido Univ., Dental Hospital, Lecturer, 歯学部・付属病院, 講師 (90200617)
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| Co-Investigator(Kenkyū-buntansha) |
KOMATSU Kosetsu Hokkaido Univ., School of Dentistry, Instructor, 歯学部, 助手 (90271668)
INOUE Nobuo Hokkaido Univ., Dental Hospital, Associate Prof., 歯学部・付属病院, 助教授 (20091415)
TOTSUYA Yasunori Hokkaido Univ., School of Dentistry, Prof., 歯学部, 教授 (00109456)
SATOH Kaoru Hokkaido Univ., Dental Hospital, Instructor, 歯学部・付属病院, 助手 (40281828)
MINOWA Kazuyuki Hokkaido Univ., School of Dentistry, Instructor, 歯学部, 助手 (30209845)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | blood flow volume / masticatory muscles / energy metabolism / 31P-MRS |
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| Research Abstract |
In order to clarify the influences of chronic decrease in blood flow volume on energy metabolism in masticatory muscles, we tried to establish the method for obtaining 31P-spectra by using NMR, and tried to make the the wister rat model in which the blood flow volume of the gastrocneminus muscles and the soleus muscles chronically continued to decrease with bound dominant artery. As the parameters of regional tissue blood flow volume, oxygen saturation level of blood (StO2) and total hemoglobin (Hb) volume were measured using a three wavelength spectrophotometric method.
The results were as follows;
1. Using a 7T NMR and a surface coil (25mm diameter), we obtained the 31P-spectra of ATP sample, and we were able to detect peaks of α, β, γ-ATP. However, the 31P-spectra of rat muscles were not obtained clearly because of noise from surrounding tissues. This problem is expected to be solved by improvement in program of control system of the NMR.
2. We obtained precise reproduciblity in measurement of StO2 and Hb volume, when we fixed both the probe of measuring apparatus and the leg of rats using devices which we made for fixation appropriately.
3. Just after binding of the artery, the value of StO2 and total Hb volume declined by about 30% and 70%, respectively. These levels continued one or two months after binding. Therefore, it was suggested that we could make the relatively chronic model in which the blood flow volume of the gastrocneminus muscle and the soleus muscles decreased obviously.
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