Study on pathogenesis of inveterate oral mucous membrane diseases : Investigation of superoxide anion generation from peripheral blood neutrophils and various plasma cytokine levels
Grant-in-Aid for Scientific Research (C).
|Research Institution||Shimane Medical University|
HARADA Toshiro School of Medicine, Shimane Medical University, Associate Professor, 医学部, 助教授 (90135921)
成相 義樹 島根医科大学, 医学部, 医員
金月 章 島根医科大学, 医学部, 医員
園山 学 島根医科大学, 医学部, 医員
松田 源 島根医科大学, 医学部, 医員
MATSUDA Hajime School of medicine, Shimane Medical University Senior Resident
NARIAI Yoshiki School of medicine, Shimane Medical University Senior Resident
|Project Fiscal Year
1998 – 2000
Completed(Fiscal Year 2000)
|Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 2000 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 1999 : ¥1,000,000 (Direct Cost : ¥1,000,000)
Fiscal Year 1998 : ¥1,100,000 (Direct Cost : ¥1,100,000)
|Keywords||Oral mucous membrane diseases / Oral lichen planus / Superoxide anion / Biscoclaurine alkaloids / Cytokines / 口腔粘膜難治性疾患 / 口腔扁平苔癬 / 活性酸素 / スーパーオキサイド / ビスコクラウリン型アルカロイド / サイトカイン / 口腔粘膜疾患|
The purpose of this study was to investigate the pathogenesis of various oral membrane diseases by examining the effects of biscoclaurine alkaloids (BA ; cepharanthin^<【○!R】>) on clinical symptoms and plasma cytokine levels, superoxide anion (O_2^-) generation from peripheral blood neutrophils in patients.
O_2^- generation was measured in terms of cytochrome C reduction, and plasma cytokine level was measured by enzyme-linked immunosorbent assay at Nihon-Igaku-Rinshokensa-Kenkyusho (Izumo City, Japan). The results were as follows :
(1) The symptoms in oral lichen planus (OLP), recurrent aphtha and radiation-induced oral mucositis were improved by the administration of BA.
(2)BA effectively reduced the O_2^- generation in OLP.Both the post-treatment period level of O_2^- generation, and the degree of clinical symptoms in patients with OLP were significantly lower (p<0.02) compared to those of the pre-treatment period.
(3) A decreasing tendency in plasma TNF-α and IL-β levels according to the clinical courses of the patients with OLP was observed when the clinical symptoms were improved.
These results suggest that the overproduction of O_2^- from peripheral blood neutrophils and various plasma cytokines such as TNF-α and IL-β may be closely related to pathogenesis of OLP.However, the pathogenesis of the other diseases such as recurrent aphtha, leukoplakia, and radiation-induced oral mucositis is still a theme of this research.
Research Output (5results)