|Budget Amount *help
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2000 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1999 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1998 : ¥2,600,000 (Direct Cost : ¥2,600,000)
For the study according to the treatment of trigeminal neuralgia, we carried out the histological and electrophysiplogical study with injecting ADM to the trigeminal nerve terminal and transporting ADM to the ganglion by retrograde axoplasmic transport.
1. Histological Study (1)Fluorescence microscopic Observation : We used female Wistar strain rats. Under general anesthesia (intraperitoneal injection of sodium pentobarbital), the inferior alveolar nerve was exposed at the mental foramen.10μ1 of a 5 %, 1 %, 0.5 %, 0.1 % concentration of ADM was injected into the nerve of each rat with a microsyringe. After 12 hours, 18 hours and 24 hours, the rat was perfused.The trigeminal ganglion was removed and quenched. 20 μm-thick sections were cut on a cryomicrotome.The specimens were examined with a fluorescence microscope to observe autofluorescence of ADM.. In result, 12 hours after 5 % ADM iniection, auto fluorescence of ADM was observed. And 18 hours after 1 % ADM mjection, weak auto fluores
cence was observed. These showed ADM was transported to the trigeminal ganglion. (2)Histopathological findings : After injection of ADM, the trigeminal ganglion was removed (after 24 hours,7 days, and 21 days) and parafin-embedded.6 μm-thick sections were stained by the hematoxylin ana eosin, Kluver- Barrera methods.7 days after injection of 5 %ADM, ganglion cells showed vacuoles, chromatolysis, and displacement of nucleus.21 days after, perikaryon was dissolved and showed basket-like form. Gangion cells showed karyopyKnosis or karyoclasis.
2. Electrophysiological study : The inferior alveolar nerve was exposed at the mental foramen : The nerve was stimulated by electric stimulation system and evoked potentials were recorded. Other rats were injected 5 % ADM to the nerve. After 24 hours, 7 days, and 21 days, the evoked potentials were recorded.24 hours after injection, evoked potentials were much lowwer than the potentials of no-injection rat. After 7days and 21days,the were form of evoked potentials were not recorded. Therefore, these results suggest that the method can destroy the trigeminal ganglion cells selectively. Less