|Budget Amount *help
¥3,100,000 (Direct Cost : ¥3,100,000)
Fiscal Year 1999 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1998 : ¥2,400,000 (Direct Cost : ¥2,400,000)
We studied the concentrations in seurm osteocalcin (s-BGP) and other serum components incidental to experimental tooth movement in adult male rats and in adult male rats bred by Ca feed and in pregnant rats in order to know systemic reactions.
1. Adult male Wistar rats were divided into three main groups [a control group c of 25 rats, an experimental tooth movement group (E) of 25 rats and an experimental no-tooth movement group (Psue) of 25 rats with only appliances]. In E group, the upper first molars were moved by wire springs for 1, 3, 5, 7 and 21 days. Serum BGP and other serum components were measured, in each group. Results : There was no significant difference among three groups except a significant difference of the BGP level between E-and Psue-groups at day 7. However, a slight increase of s-BGP levels in E-group was characterized in each experimental period in compared with those of C-and Psue-groups.
2. Eight weeks old male Wistar rats were divided into four main groups [a co
ntrol group bred with CaィイD1+ィエD1 feed c of 20 rats, an experimental tooth movement group with CaィイD1+ィエD1 feed (E) of 20 rats, a group bred with CaィイD1-ィエD1 feed (CaィイD1-ィエD1) of 20 rats and an experimental tooth movement group with CaィイD1-ィエD1 feed (Ca+E) of 20 rats]. In E- and Ca+E- groups, the upper first molars were moved by wire springs for 1, 3, 5 and 7 days. Serum BGP and other serum components were measured, in each group. Results : In each experimental stage, the values of s-BGP were higher levels in Ca- and Ca+E -groups than in C- and E-groups. On day 3, the values of s-BGP and s-Alk-p were lower levels in E- and Ca+E -groups than in C- and Ca-groups.
3. Adult female Wistar rats were divided into three main groups : NP-group (not pregnant, 18 rats), P-group (pregnant, 18 rats) and PE-group (pregnancy + tooth movement, 18 rats). In PE-group, the lower first molars were moved for 7, 14, 21 days. Serum BGP and other serum components were measured, in each group. Results : The values of the s-components of the P- and PE-groups versus NP-group (except s-BGP, s-total CHO and total protein) showed significant decreases at day 21. In s-Ca, there was significant difference at day 21 between the P- and PE-groups. Less